1. 毕业设计（论文）的内容和要求
论文内容 基质金属蛋白酶（matrix metalloproteinases，简称MMPs）是一类以Zn2 为辅因子具有高度同源性的内肽酶，已发现至少28 种，与人体直接相关的有23种，是细胞外基质（ECM）和基底膜降解主要酶类之一，具有催化、调节、导向、锚定、胞内应答、防御、进攻等细胞功能。
与机体的老化和功能减退、亚健康状态、头晕、缺血、心脑血管疾病、高血压、关节炎、癌症、HIV、肠类疾病、皮肤美容、组织清创、细胞及组织分离等方面相关。
本研究拟将从鲫鱼中提取的基质金属蛋白酶粗酶进行纯化，并对纯化后的蛋白酶进行酶学特性分析。
2. 参考文献
[1] A. L. Phillips, W. J. Means, N. Kalchayanand, R. J. McCormick and K. W. Miller. Bovine placental protease specificity toward muscle connective tissue proteins. Journal Of Animal Science，2000,78:1861~1866.[2] David D. DEAN and J. Frederick WOESSNER, JR.. Extracts of human articular cartilage contain an inhibitor of tissue metalloproteinases. Biochemistry Journal，1984,218,277~280.[3] Das, S., M. Mandal, et al. . Identification, purification and characterization of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle plasma membrane. Molecular and Cellular Biochemistry, 2004,258(1-2): 73-89.[4] Indra, D., K. Ramalingam, et al. Isolation, purification and characterization of collagenase from hepatopancreas of the land snail Achatina fulica. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2005,142(1): 1-7.[5] Morodomi, T., Y. Ogata, et al.Purification and characterization of matrix metallproteinase-9 from U937 monocytic leukemia and ht1080 fibrosarcoma cells . Biochemical Journal, 1992,285: 603-611.
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