1. 毕业设计（论文）的内容和要求
1. 对重组构建的新型褐藻胶裂解酶进行同源建模及蛋白序列分析2. 对获得的褐藻胶裂解酶进行酶学性质表征并探究结构域重组前后其功能变化3. 完成南京工业大学本科生毕业论文要求
2. 参考文献
1. Zhu B, Yin H. Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications[J]. Bioengineered, 2015, 6(3):125-131.2. Yu Z, Zhu B, Wang W, et al. Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp[J]. International Journal of Biological Macromolecules, 2018, 112:937.3. Huang G, Wen S, Liao S, et al. Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2[J]. Biotechnology Letters, 2019, 41(1).4. Jagtap, S. S, Hehemann, J.-H, Polz, M. F, et al. Comparative Biochemical Characterization of Three Exolytic Oligoalginate Lyases from Vibrio splendidus Reveals Complementary Substrate Scope, Temperature, and pH Adaptations[J]. Applied Environmental Microbiology, 80(14):4207-4214.5. Kim H T, Chung J H, Wang D, et al. Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans2-40[J]. Applied Microbiology Biotechnology, 2012, 93(5):2233-2239.6. Park H H, Kam N, Lee E Y, et al. Cloning and Characterization of a Novel Oligoalginate Lyase from a Newly Isolated BacteriumSphingomonassp. MJ-3[J]. Marine Biotechnology, 2012, 14(2):189-202.7. Park D, Jagtap S, Nair S K. Structure of a PL17 Family Alginate Lyase Demonstrates Functional Similarities Among Exotype Depolymerases[J]. Journal of Biological Chemistry, 2014, 289(12).8. Zedong, Jiang, Yuxi, et al. Molecular cloning and characterization of AlgL17, a new exo-oligoalginate lyase from Microbulbifer sp. ALW1.[J]. Protein expression and purification, 2019.9. Wang L, Li S, Yu W, et al. Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.[J]. Biotechnology Letters, 2014, 37(3):665-71.
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