1. 毕业设计（论文）的内容和要求
制定实验方案，具体内容和步骤如下：1)饲养小鼠并学会口服灌胃和腹腔注射给药；2)用雷公藤甲素进行干预，14天后解刨小鼠测定相关指标；3)实验数据分析和处理，对雷公藤甲素的生殖毒性进行检测。
本研究以ICR小鼠作为研究对象，用雷公藤甲素进行诱导，考察该药物活性成分对生殖器官附睾的毒性作用，并通过生化指标检测和免疫组化，探究毒性产生的可能机制，最终根据实验数据进行分析处理得出相应结论。
2. 参考文献
[1] 国家中医药管理局《中华本草》编委会． 中华本草． 第五册十三卷［M］． 上海: 上海科学技术出版社，1999:206-214．[2] CHEN B J．Triptolide，a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F[ J].Leuk Lymphoma,2001,42( 3) : 253-265．[3] LI X J,JIANG Z Z,ZHANG L Y．Triptolide: progress on research in pharmacodynamics and toxicology ［J］.J Ethnopharmacol，2014，155( 1) : 67-79．[4] WANG X , ZHAO F , LV Z M. Triptolide disrupts the actin-based Sertoli-germ cells adherens junctions by inhibiting Rho GTPases expression. Toxicology and Applied Pharmacology .310 (2016) 3240.[5] HU J Y, QIN W,ZHAO F.Protection of Quercetin against Triptolide-induced apoptosis by suppressing oxidative stress in rat Leydig cells[J].CHEMICO-BIOLOGICAL INTERACTIONS.2015：38-46. [6] PUIGSERVER P, SPIEGELMAN B M..Peroxisome proliferator-activated receptorgamma coactivator 1 alpha (PGC-1 alpha): transcriptional coactivator and metabolic regulator[J]. Endocrine Reviews. Rev. 24 (2003) 78-90.[7]RHEE J, INOUE Y, . YOON J C, et al. Spiegelman, Regulation of hepatic fasting response by PPARgamma coactivator-1alpha (PGC-1): requirement for hepatocyte nuclear factor 4alpha in gluconeogenesis[J]. Proceedings of the National Academy of Sciences of the United States of America.100 (2003) 4012-4017.[8] JULIE S P,STAVIT D,MARC U. Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators, Cell 127 (2006) 397-408.[9] PABLO J F M, AUWERX J. Regulation of PGC-1alpha, a nodal regulator of mitochondrial biogenesis[J]. The American journal of clinical nutrition. 93 (2011), 884S-890. [10] J. St-Pierre, S. Drori, M. Uldry, J.M. Silvaggi, J. Rhee, S. Jager, C. Handschin,K. Zheng, J. Lin, W. Yang, D.K. Simon, R. Bachoo, B.M. Spiegelman, Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators, Cell 127 (2006) 397-408.[11] P.J. Fernandez-Marcos, J. Auwerx, Regulation of PGC-1alpha, a nodal regulator of mitochondrial biogenesis, Am. J. Clin. Nutr. 93 (2011), 884S-890.[12] MA B,QI H H,LI J.Triptolide disrupts fatty acids and peroxisome proliferator-activated receptor (PPAR) levels in male mice testes followed by testicular injury: A GC-MS based metabolomics study[J].TOXICOLOGY.2015：84-95.[13] 孟祥英,乔晋娟,赵荣兰,伊正君等. DNA 氧化损伤标志物 8-OHdG 检测方法及其生物学应用. 中国卫生检验杂志 2018 年 10 月第 28 卷第 19 期. [14] HRUSZKEWYCZ AM,BERGTOLD DS.The 8-Hydroxyguanine content of isolated mitochondria increases with lipid-peroxidation［J］. Mutation Research Letters,1990,244( 2) : 123-128．[13] Hruszkewycz AM,Bergtold DS.The 8-Hydroxyguanine content of isolated mitochondria increases with lipid-peroxidation［J］.Mutat Res,1990,244( 2) : 123-128．[15] WANG XW;TIAN RM;YANG YQ.Triptriolide antagonizes triptolide-induced nephrocyte apoptosis via inhibiting oxidative stress in vitro and in vivo.[J].Biomedicine Pharmacotherapy = Biomedecine Pharmacotherapie.2019：109232.[16]Bo Ma, Jie Zhang, Zhiming Zhu, Luteolin ameliorates testis injury and blood-testis barrier disruption through the Nrf2 signaling pathway and by up-regulating Cx43. Molecular Nutrition Food Research.201800843. [17]P.J. Fernandez-Marcos, J. Auwerx, Regulation of PGC-1alpha, a nodal regulator of mitochondrial biogenesis, Am. J. Clin. Nutr. 93 (2011), 884-890.
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