1. 毕业设计（论文）的内容和要求
课题简介糖基转移酶Yjic是从实验室保藏的枯草芽孢杆菌中筛选获得的UDP糖基转移酶。
该酶以pET28a为表达载体，E.coli BL21为宿主菌，经IPTG诱导可实现高效可溶表达。
研究表明Yjic具有催化PPD合成稀有人参皂苷Rh2的能力。
2. 参考文献
[1] Z.B. Dai, B.B. Wang, Y. Liu, M.Y. Shi, D. Wang, X.N. Zhang, T. Liu, L.Q. Huang, X.L.Zhang, Producing aglycons of ginsenosides in bakers yeast, Sci. Rep. 4 (2014) 6.[2] L. Li, S.-Y. Shin, S.J. Lee, J.S. Moon, W.T. Im, N.S. Han, Production of GinsenosideF2 by Using Lactococcus lactis with Enhanced Expression of beta-GlucosidaseGene from Paenibacillus mucilaginosus, J. Agric. Food. Chem. 64 (2016) 25062512.[3] J. Pei, A. Chen, L. Zhao, F. Cao, G. Ding, W. Xiao, Enzymatic transformation ofginsenoside Rb1 to ginsenoside 20(S)-Rg3 by GH3 b-glucosidase fromThermotoga thermarum DSM 5069 T, J. Agric. Food. Chem. 65 (2017) 60426048.[4] J.Y. Seo, S.H. Ju, J. Oh, S.K. Lee, J.-S. Kim, Neuroprotective and cognition-enhancing effects of compound K isolated from red ginseng, J. Agric. Food.Chem. 64 (2016) 28552864.[5] L.P. Christensen, Ginsenosides chemistry, biosynthesis, analysis, and potentialhealth effects, Adv. Food Nutr. Res. 55 (2009) 199.[6] X. Jin, D.B. Che, Z.H. Zhang, H.M. Yan, Z.Y. Jia, X.B. Jia, Ginseng consumption andrisk of cancer: a meta-analysis, J. Ginseng Res. 40 (2016) 269277.[7] M.K. Choo, E.K. Park, M.J. Han, D.H. Kim, Antiallergic activity of ginseng and itsginsenosides, Planta Medica 69 (2003) 518522.[8] G.-W. Liu, Y.-H. Liu, G.-S. Jiang, W.-D. Ren, The reversal effect of GinsenosideRh2 on drug resistance in human colorectal carcinoma cells and itsmechanism, Hum. Cell 31 (2018) 189198.[9] X.W. Xie, A. Eberding, C. Madera, L. Fazli, W. Jia, L. Goldenberg, M. Gleave, E.S.Guns, Rh2 synergistically enhances paclitaxel or mitoxantrone in prostatecancer models, J. Urol. 175 (2006) 19261931.[10] J. Liao, J. Sun, Y. Niu, B. Yu, Synthesis of ginsenoside Rh2 andchikusetsusaponin-LT8 via gold(I)-catalyzed glycosylation with a glycosylortho-alkynylbenzoate as donor, Tetrahedr. Lett. 52 (2011) 30753078.[11] J. Yu, J. Sun, Y. Niu, R. Li, J. Liao, F. Zhang, B. Yu, Synthetic access toward thediverse ginsenosides, Chem. Sci. 4 (2013) 38993905.[12] Y.J. Kim, D. Zhang, D.C. Yang, Biosynthesis and biotechnological production ofginsenosides, Biotechnol. Adv. 33 (2015) 717735.[13] C. Zhao, X. Gao, X. Liu, Y. Wang, S. Yang, F. Wang, Y. Ren, Enhancingbiosynthesis of a ginsenoside precursor by self-assembly of two key enzymesin Pichia pastoris, J. Agric. Food. Chem. 64 (2016) 33803385.[14] Z. Dai, Y. Liu, X. Zhang, M. Shi, B. Wang, D. Wang, L. Huang, X. Zhang, Metabolicengineering of Saccharomyces cerevisiae for production of ginsenosides,Metab. Eng. 20 (2013) 146156.
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