1. 毕业设计(论文)的内容和要求
酶是一种具有高效性、选择性和特异性的绿色生物催化剂 ,在食品、医疗、能源等方面都有广泛应用。
传统的固定化方法具有适用范围窄、酶活性降低、酶回收率低等缺点。
针对上述问题,研究人员在传统固定化方法的基础上,结合新型固定载体材料和固定方式研究出新型酶固定化技术。
2. 参考文献
[1] Flores G, Sobern X, Osuna J. Production of a fully functional, permuted single-chain penicillin G acylase[J]. Protein Science A Publication of the Protein Society, 2004, 13(6):1677-1683. [2] 崔鹏, 万敏. 青霉素酰化酶的生产与应用新进展[J]. 能源化工, 2005, 26(6):42-45. [3] 杨正. 青霉素酰化酶的分离纯化和固定化研究新进展[J]. 安徽化工, 2010, 36(3):7-10. [4] 安蔚. 酶法合成头孢氨苄过程中固定化青霉素酰化酶酶活稳定性及其再生研究[D]. 河北科技大学, 2016. [5] 陆健美. 合成阿莫西林用青霉素酰化酶在大肠杆菌中的表达[D]. 北京化工大学, 2013. [6] Romero O, Guisn J M, Illanes A, et al. Reactivation of penicillin acylase biocatalysts: Effect of the intensity of enzyme-support attachment and enzyme load[J]. Journal of Molecular Catalysis B Enzymatic, 2012, 74(3):224-229. [7] Chandel A K, Rao L V, Narasu M L, et al. The realm of penicillin G acylase in β-lactam antibiotics[J]. Enzyme Microbial Technology, 2008, 42(3):199-207. [8] 鲜海军, 王祯祥. 以聚丙烯腈纤维为载体制备固定化青霉素G酰化酶的研究[J]. 微生物学报, 2001, 41(4):475-480. [9] Tidwell T T. Hugo (Ugo) Schiff, Schiff bases, and a century of beta-lactam synthesis.[J]. Angewandte Chemie, 2008, 47(6):1016-20. [10] Arroyo M, De l M I, Acebal C, et al. Biotechnological applications of penicillin acylases: state-of-the-art.[J]. Applied Microbiology Biotechnology, 2003, 60(5):507-14. [11] Sheldon R A. Enzyme Immobilization: The Quest for Optimum Performance[J]. Cheminform, 2007, 38(36):no-no. [12] Aytar B S, Bakir U. Preparation of cross-linked tyrosinase aggregates[J]. Process Biochemistry, 2008, 43(2):125-131. [13] Kim M I, Kim J, Lee J, et al. One-dimensional crosslinked enzyme aggregates in SBA-15: Superior catalytic behavior to conventional enzyme immobilization[J]. Microporous Mesoporous Materials, 2008, 111(1):18-23. [14] Wilson L, Illanes A, Pessela B C, et al. Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: evaluation of a novel biocatalyst in organic media.[J]. Biotechnology Bioengineering, 2004, 86(5):558-62.
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