1. 毕业设计（论文）的内容和要求
1. 培养基优化优化假单胞菌lk08的发酵培养基组分，完成响应面分析。
2. 环境条件优化改变pH、温度等环境因素，考察不同条件下1,5-二羟基萘的合成效率，以及微生物生长量。
要求：数据详实有效，说明有理有据。
2. 参考文献
Chauhan, A., Fazlurrahman., Oakeshott, J. G. and Jain, R. K. (2008). Bacterial metabolism of polycyclic aromatic hydrocarbons: Strategies for bioremediation. Indian J Microbiol., 48(1); 95-113. Chen, B., Huang, J., Yuan, K., Lin, L., Wang, X., Yang, L., et al. (2015). Direct evidences on bacterial growth pattern regulating pyrene degradation pathway and genotypic dioxygenase expression. Mar. Pollut. Bull., 105(1); 73-80.Chupungars, K., Rerngsamran, P. and Thaniyavarn, S. (2009). Polycyclic aromatic hydrocarbons degradation by Agrocybe sp. CU-43 and its fluorene transformation. Int Biodet Biodeg., 63(1); 93-99.Drainas, C. and Koukkou, A. I. (2007). Taxonomic identification, phenanthrene uptake activity, and membrane lipid alterations of the PAH degrading. Appl Microbiol Biotechnol., 76; 709-717.Fooladi, T., Moazami, N., Abdeshahian, P., Kadier, A., Ghojavand, H., Wan Yusoff, W. M. and Hamid, A. A. (2016). Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field. J Pet Sci Eng., 145; 510-519. Gupta, B., Puri, S. and Kaur, J. (2016). Isolation and characterization of pyrene degrading bacteria from petroleum oil contaminetd soil from Chandigarh. Int J Biol Pharm Allied Sci., 5(9); 2084-2096. Haritash, A. K. and Kaushik, C. P. (2009). Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): A review. J Hazard Mater., 169(1-3); 1-15. Holt, J. H., Krieg, N. R., Sneath, P. H. A., Staley, J.. and Williams, S.. (14). Bergeys manual of determinative bacteriology ninth edition. European journal of paediatric neurology: EJPN: official journal of the European Paediatric Neurology Society., 13; 560. Kasumba., J. and Holmen., B. A. (2018). Heterogeneous ozonation reactions of PAHs andfatty acid methyl esters in biodiesel particulate matter. Atmos. Environ.,175; 15-24.
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