1. 毕业设计（论文）的内容和要求
（1）掌握文献查阅的一般方法，学会在中国期刊网、Web of Science科学引文索引、Springer Link电子期刊、Elsevier SDOS电子期刊等检索资源上查阅关于固定化细胞发酵和大豆生物炼制等的相关文献，并对芽孢杆菌发酵培养有全面了解。
（2）文献阅读及综述：阅读与课题相关的中英文文献，了解国内外的研究动态，撰写文献综述。
（3）明确实验任务，拟定实验方案：根据所查阅文献的内容，确定研究内容及方案，拟定科学可行的研究计划。
2. 参考文献
[1] Ebrahiminezhad A, Varma V, Yang S, et al. Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation[J]. Applied microbiology and biotechnology, 2016, 100(1): 173-180.[2] Mulla S I, Bangeppagari M, Mahadevan G D, et al. Biodegradation of 3-chlorobenzoate and 3-hydroxybenzoate by polyurethane foam immobilized cells of Bacillus sp. OS13[J]. Journal of environmental chemical engineering, 2016, 4(2): 1423-1431.[3] Ma Z, Li C, Su H. Dark bio-hydrogen fermentation by an immobilized mixed culture of Bacillus cereus and Brevumdimonas naejangsanensis[J]. Renewable energy, 2017, 105: 458-464.[4] Wang J, Hu Y, Qiu C, et al. Immobilized Cells of Bacillus circulans ATCC 21783 on Palm Curtain for Fermentation in 5 L Fermentation Tanks[J]. Molecules, 2018, 23(11): 2888.[5] Ke Q, Zhang Y, Wu X, et al. Sustainable biodegradation of phenol by immobilized Bacillus sp. SAS19 with porous carbonaceous gels as carriers[J]. Journal of environmental management, 2018, 222: 185-189.[6] Zhang Y, Li X, Zhang C, et al. Production of d-alanine from dl-alanine using immobilized cells of Bacillus subtilis HLZ-68[J]. World Journal of Microbiology and Biotechnology, 2017, 33(9): 176.[7] Kureel M K, Geed S R, Giri B S, et al. Biodegradation and kinetic study of benzene in bioreactor packed with PUF and alginate beads and immobilized with Bacillus sp. M3[J]. Bioresource technology, 2017, 242: 92-100.[8] Yi G, Liu Q, Lin J, et al. Repeated batch fermentation for surfactin production with immobilized Bacillus subtilis BS‐37: two‐stage pH control and foam fractionation[J]. Journal of Chemical Technology I, Ribeiro M C, dos Santos Jnior S R, et al. Production of cyclodextrin glycosyltransferase by immobilized Bacillus sp. on chitosan matrix[J]. Bioprocess and biosystems engineering, 2016, 39(10): 1487-1500.[11] Feng X, Tang B, Jiang Y, et al. Efficient production of poly‐γ‐glutamic acid from cane molasses by Bacillus subtilis NX‐2 immobilized on chemically modified sugarcane bagasse[J]. Journal of Chemical Technology Chitosan Complex and Its Application for Nattokinase Fermentation[J]. International Journal of Pharmaceutical Science Invention, 2016, 30.[14] Kureel M K, Geed S R, Giri B S, et al. Removal of aqueous benzene in the immobilized batch and continuous packed bed bioreactor by isolated Bacillus sp. M1[J]. Resource-Efficient Technologies, 2016, 2: S87-S95.[15] Felshia S C, Karthick N A, Thilagam R, et al. Efficacy of free and encapsulated Bacillus lichenformis strain SL10 on degradation of phenol: A comparative study of degradation kinetics[J]. Journal of environmental management, 2017, 197: 373-383.[16] Guleria S, Walia A, Chauhan A, et al. Immobilization of Bacillus amyloliquefaciens SP1 and its alkaline protease in various matrices for effective hydrolysis of casein[J]. 3 Biotech, 2016, 6(2): 208.[17] Jiang Y, Tang B, Xu Z, et al. Improvement of poly-γ-glutamic acid biosynthesis in a moving bed biofilm reactor by Bacillus subtilis NX-2[J]. Bioresource technology, 2016, 218: 360-366.[18] Hassan S W, Ali M, Elsayed S M. Production of cold active lipase by free and immobilized marine Bacillus cereus HSS: Application in wastewater treatment[J]. Frontiers in microbiology, 2018, 9: 2377.[19] Ran G Q, Tan D, Dai W E, et al. Immobilization of alkaline polygalacturonate lyase from Bacillus subtilis on the surface of bacterial polyhydroxyalkanoate nano-granules[J]. Applied microbiology and biotechnology, 2017, 101(8): 3247-3258.[20] Oliveira A F, Bastos R G, Lucimara G. Bacillus subtilis immobilization in alginate microfluidic-based microparticles aiming to improve lipase productivity[J]. Biochemical engineering journal, 2019, 143: 110-120.[21] Wu B, Yu Q, Chang S, et al. Expansin assisted bio-affinity immobilization of endoxylanase from Bacillus subtilis onto corncob residue: Characterization and efficient production of xylooligosaccharides[J]. Food chemistry, 2019, 282: 101-108.[22] Mahdinia E, Demirci A, Berenjian A. Implementation of fed-batch strategies for vitamin K (menaquinone-7) production by Bacillus subtilis natto in biofilm reactors[J]. Applied microbiology and biotechnology, 2018, 102(21): 9147-9157.[23] Prakash J, Gupta R K, Priyanka X X, et al. Bioprocessing of biodiesel industry effluent by immobilized bacteria to produce value-added products[J]. Applied biochemistry and biotechnology, 2018, 185(1): 179-190.[24] Taskin M, Ucar M H, Unver Y, et al. Lipase production with free and immobilized cells of cold-adapted yeast Rhodotorula glutinis HL25[J]. Biocatalysis and Agricultural Biotechnology, 2016, 8: 97-103.[25] Seifan M, Samani A K, Hewitt S, et al. The effect of cell immobilization by calcium alginate on bacterially induced calcium carbonate precipitation[J]. Fermentation, 2017, 3(4): 57.[26] Wen X, Du C, Zeng G, et al. A novel biosorbent prepared by immobilized Bacillus licheniformis for lead removal from wastewater[J]. Chemosphere, 2018, 200: 173-179.[27] Bouabidi Z B, El-Naas M H, Zhang Z. Immobilization of microbial cells for the biotreatment of wastewater: A review[J]. Environmental Chemistry Letters, 2019, 17(1): 241-257.[28] Xue J, Wu Y, Shi K, et al. Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment[J]. Bioresource technology, 2019, 280: 88-94.
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