1. 毕业设计(论文)的内容和要求
本实验基于二氧化硅的优异性能,合成了氨基化树枝状介孔二氧化硅纳米材料,并以其为载体构建多酶纳米反应器。
具体的研究工作如下:1.使用十六烷基三甲基溴化铵、水杨酸钠作为结构导向剂,原硅酸四乙酯作为硅源和三乙醇胺作为催化剂合成枝状介孔硅,并进行相关表征。
利用戊二醛交联法将GOX,HRP分别固定在氨基化枝状介孔硅里。
2. 参考文献
1. Yang Y, Bernardi S, Song H, et al. Anion assisted synthesis of large pore hollow dendritic mesoporous organosilica nanoparticles: understanding the composition gradient[J]. Chemistry of Materials, 2016, 28(3): 704-707.2. Fang X, Duan Y, Liu Y, et al. Photochemical Bionanoreactor for Efficient Visible-Light-Driven in Vitro Drug Metabolism[J]. Analytical chemistry, 2017, 89(14): 7365-7372.3. Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976, 72(1-2): 248-254.4. Lei C, Xu C, Nouwens A, et al. Ultrasensitive ELISA enhanced by dendritic mesoporous silica nanoparticles[J]. Journal of Materials Chemistry B, 2016, 4(29): 4975-4979.5. Smeller L, Meersman F, Fidy J, et al. High-pressure FTIR study of the stability of horseradish peroxidase. Effect of heme substitution, ligand binding, Ca removal, and reduction of the disulfide bonds[J]. Biochemistry, 2003, 42(2): 553-561.6. Cao X, Li Y, Zhang Z, et al. Catalytic activity and stability of glucose oxidase/horseradish peroxidase co-confined in macroporous silica foam[J]. Analyst, 2012, 137(24): 5785-5791.7. Fu J, Liu M, Liu Y, et al. Interenzyme substrate diffusion for an enzyme cascade organized on spatially addressable DNA nanostructures[J]. Journal of the American Chemical Society, 2012, 134(12): 5516-5519.8. Li Y Q, Guan L Y, Zhang H L, et al. Distance-dependent metal-enhanced quantum dots fluorescence analysis in solution by capillary electrophoresis and its application to DNA detection[J]. Analytical chemistry, 2011, 83(11): 4103-4109.9. Khan M, Park S Y. Glucose biosensor based on GOx/HRP bienzyme at liquidcrystal/aqueous interface[J]. Journal of colloid and interface science, 2015, 457: 281-288.10. Qu Z, Xu H, Xu P, et al. Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels[J]. Analytical chemistry, 2014, 86(19): 9367-9371.11. Huang Y, Lin Y, Ran X, et al. Self‐Assembly and Compartmentalization of Nanozymes in Mesoporous Silica‐Based Nanoreactors[J]. ChemistryA European Journal, 2016, 22(16): 5705-5711.12. Hu Y, Grsche M, Sheshachala S, et al. Bottom‐Up Assembly of DNASilica Nanocomposites into Micrometer‐Sized Hollow Spheres[J]. Angewandte Chemie International Edition, 2019.13. Delvaux M, Walcarius A, Demoustier-Champagne S. Bienzyme HRPGOx-modified gold nanoelectrodes for the sensitive amperometric detection of glucose at low overpotentials[J]. Biosensors and Bioelectronics, 2005, 20(8): 1587-1594.14. Li Y, Deng J, Fang L, et al. A novel electrochemical DNA biosensor based on HRP-mimicking hemin/G-quadruplex wrapped GOx nanocomposites as tag for detection of Escherichia coli O157: H7[J]. Biosensors and Bioelectronics, 2015, 63: 1-6.15. Liao C A, Wu Q, Wei Q C, et al. Bioinorganic Nanocomposite Hydrogels Formed by HRPGOx‐Cascade‐Catalyzed Polymerization and Exfoliation of the Layered Composites[J]. ChemistryA European Journal, 2015, 21(36): 12620-12626.16. Li Z, Zhang Y, Su Y, et al. Spatial co-localization of multi-enzymes by inorganic nanocrystalprotein complexes[J]. Chemical Communications, 2014, 50(83): 12465-12468.17. Razzaghi M, Karimi A, Aghdasinia H, et al. Oxidase-Peroxidase sequential polymerization for removal of a dye from contaminated water by horseradish peroxidase (HRP)/glucose oxidase (GOx)/polyurethane hybrid catalyst[J]. Korean Journal of Chemical Engineering, 2017, 34(11): 2870-2878.18. Zhao Z, Fu J, Dhakal S, et al. Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion[J]. Nature communications, 2016, 7: 10619.19. Zhang Y, Lyu F, Ge J, et al. Ink-jet printing an optimal multi-enzyme system[J]. Chemical Communications, 2014, 50(85): 12919-12922.20. Razzaghi M, Karimi A, Ansari Z, et al. Phenol removal by HRP/GOx/ZSM-5 from aqueous solution: Artificial neural network simulation and genetic algorithms optimization[J]. Journal of the Taiwan Institute of Chemical Engineers, 2018, 89: 1-14.
以上是毕业论文任务书,课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。