全文总字数:1413字
1. 毕业设计(论文)的内容和要求
1、基因克隆及载体的构建 2、农杆菌的转化 3、转基因拟南芥的获得4、转基因拟南芥植株的鉴定5、转基因拟南芥的筛选6、转基因拟南芥的耐逆性鉴定6.1 形态指标测定(1)种子萌发率统计(2)根长测定6.2 生理指标测定(1)丙二酸(MDA)含量的测定(2)质膜相对透性测定(3) 相对含水量测定
2. 实验内容和要求
1、构建ZmWRKY62过表达转基因拟南芥 2、对ZmWRKY62转基因拟南芥进行抗逆性分析及作用机理的初步分析
3. 参考文献
SONG X H, XU Y, LI G M, et al. Isolation, characterization of Phodococcus sp. P14 capable of degrading high-molecularweight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons[J]. Marine Pollution Bulletin, 2011, 62(10): 2122-2128.rskove R, Mcdonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J]. The Journal of Agricultural Science, 1979,Menke K H, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research Development, 1988, 28: 47-55. Viola E, Zimbardi P, Cardinale M, et a1. Processing cereal straws by steam explosion in a pilot plant to enhance digestibility in ruminants[J]. Bioresource Technology 2008,99(4):681-689. He L W, Meng Q X, Li D Y, et a1. Effect of steam explosion on in vitro gas production kinetics and rumen fermentation profiles of three common straws[J]. Italian Journal of Animal Science, 2015, 14(4): 727-733.
4. 毕业设计(论文)计划
1月-2月,开题报告的撰写及实验方案设计,写出论文大纲 2月-3月,完成菌株的筛选,培养条件的优化 3月-4月,制备缓释制剂及数据处理 4月-5月,论文撰写
以上是毕业论文任务书,课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。