全文总字数：4129字
1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解萜类化合物功能、解脂耶氏酵母合成萜类的途径及改造方法，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
实验内容：萜类化合物是一种自然界中广泛存在的天然萜烯化合物，具有多种应用价值，例如抗菌消炎、抗癌活性等药用价值，也可作为新型航空燃料等。
合成萜类化合物的途径主要有MEP和MVA途径，其中MVA途径相比MEP途径具有更高的前体通量。
解脂耶氏酵母作为一种含油酵母，具有内源MVA途径，是生产萜类化合物的有潜力的生产菌株。
3. 参考文献
5、参考文献：（10篇）Liu, Yinghang. Jiang, Xin. Cui, Zhiyong. Wang, Zhaoxuan. Qi, Qingsheng ,Hou, J. 2019. Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene. Biotechnology for Biofuels 12(1).Zu, Y., Prather, K. L., Stephanopoulos, G. 2020 . Metabolic engineering strategies to overcome precursor limitations in isoprenoid biosynthesis. Curr Opin Biotechnol 66,171-178.Becker, J., Wittmann, C. 2015. Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. Angew. Chem. Int. Ed. Engl., 54(11), 3328-50.Bouvier, F., Rahier, A., Camara, B. 2005. Biogenesis, molecular regulation and function of plant isoprenoids. Progress in lipid research, 44(6), 357-429.Cao, X., Lv, Y.B., Chen, J., Imanaka, T., Wei, L.J., Hua, Q. 2016. Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction. Biotechnol Biofuels, 9, 214.Cao, X., Wei, L.J., Lin, J.Y., Hua, Q. 2017. Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica. Bioresour Technol.Gao, S., Tong, Y., Zhu, L., Ge, M., Zhang, Y., Chen, D., Jiang, Y., Yang, S. 2017. Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous beta-carotene production. Metab Eng, 41, 192-201.Kildegaard, K.R., Adiego-Perez, B., Domenech Belda, D., Khangura, J.K., Holkenbrink, C., Borodina, I. 2017. Engineering of Yarrowia lipolytica for production of astaxanthin. Synth Syst Biotechnol, 2(4), 287-294.Liao, P., Hemmerlin, A., Bach, T.J., Chye, M.-L. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology advances, 34(5), 697-713.Nielsen, J., Keasling, Jay D. 2016. Engineering Cellular Metabolism. Cell, 164(6), 1185-1197.Paddon, C., Westfall, P.J., Pitera, D., Benjamin, K., Fisher, K., McPhee, D., D Leavell, M., Tai, A., Main, A., Eng, D., R Polichuk, D., H Teoh, K., Reed, D., Treynor, T., Lenihan, J., Fleck, M., Bajad, S., Dang, G., Diola, D., Newman, J. 2013. High-level semi-synthetic production of the potent antimalarial artemisinin.Daletos, G.，Katsimpouras, C.，Stephanopoulos, G. 2020. Novel Strategies and Platforms for Industrial Isoprenoid Engineering. Trends Biotechnol 38(7): 811-822.Roberts, S.C. 2007. Production and engineering of terpenoids in plant cell culture. Nature chemical biology, 3(7), 387-395.Rohdich, F., Hecht, S., Grtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A., Eisenreich, W. 2002. Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein. Proceedings of the National Academy of Sciences, 99(3), 1158-1163.Yang, X., Nambou, K., Wei, L., Hua, Q. 2016. Heterologous production of α-farnesene in metabolically engineered strains of Yarrowia lipolytica. Bioresour Technol, 216(2016), 1040-8.Zhao, C., Gao, Q., Chen, J., Wei, L., Imanaka, T., Hua, Q. 2017. Metabolomic changes and metabolic responses to expression of heterologous biosynthetic genes for lycopene production in Yarrowia lipolytica. Journal of Biotechnology, 251, 174-185.
4. 毕业设计（论文）计划
2020.12.1-2020.12.30 确定毕业设计的课题及相关资料的查找2020.12.31-2020.1.28 实验方案的设计2020.1.29-2020.2.28 实验材料的准备2020.2.28-2020.5.14 准备分子实验2020.5.15-2020.5.19 实验数据处理与结果分析2020.5.20-2020.6.3毕业论文书写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
