全文总字数:1717字
1. 毕业设计(论文)的内容和要求
对agr系统四个失活基因的菌株加上原始菌株进行生物膜、生理、芽孢形成、鞭毛运动、ABE发酵等一系列表征,需要做出对比,并且初步解释其原因和原理
2. 实验内容和要求
对agr系统四个失活基因的菌株加上原始菌株进行生物膜、生理、芽孢形成、鞭毛运动、ABE发酵等一系列表征,需要做出对比,并且初步解释其原因和原理
3. 参考文献
[ 1 ] 伦世仪.生化工程[M].2 版.北京:中国轻工业出版社,2008.[ 2 ] LI Z J, CHEN Y, LIU D, et al. Involvement of glycolysis/gluconeogenesis and signaling regulatory pathways inSaccharomyces cerevisiae biofilms during fermentation[J]. FrontMicrobiol,2015,DOI:10.3389/ fmicb.2015.00139.[ 3 ] LIU D,CHEN Y,LI A,et al.Enhanced butanol production bymodulation of electron flow in Clostridium acetobutylicum B3immobilized by surface adsorption[J].Bioresour Technol,2013,129:321328.[ 4 ] RAMAKRISHNA S, PRAKASHAM R. Microbial fermentationswith immobilized cells[J].Curr Sci,1999,77(1):87100.[ 5 ] TALABARDON M, YANG S T. Production of GFP andglucoamylase by recombinant Aspergillus niger: effects offermentation conditions on fungal morphology and proteinsecretion[J].Biotechnol Prog,2005,21(5):13891400.[ 6 ] HUI Y S,AMIRUL A,YAHYA A R,et al.Cellulase production byfree and immobilized Aspergillus terreus [ J]. World J MicrobBiotechnol,2010,26(1):79.[ 7 ] ABDELLA A,MAZEED T E S,ELBAZ A F,et al.Production ofβglucosidase from wheat bran and glycerol by Aspergillus niger instirred tank and rotating fibrous bed bioreactors [ J]. ProcessBiochem,2016,51(10):13311337.[ 8 ] COSTERTON J W,GEESEY G,CHENG K.How bacteria stick[J].Sci Am,1978,238(1):8695.[ 9 ] FLEMMING H C,WINGENDER J. The biofilm matrix[J]. NatRev Microbiol,2010,8(9):623.[10] SAUER K.The genomics and proteomics of biofilm formation[J].Genom Biol,2003,4(6):219.
4. 毕业设计(论文)计划
1、活化原始菌 4株突变菌2、做ABE发酵实验3、做鞭毛运动实验4、做芽孢形成实验5、生物膜形成实验
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