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1. 毕业设计（论文）的内容和要求
1、阅读本领域相关文献10-20篇；2、设计并合成不同类型的人工PLP辅因子5种；3、3种转氨酶的表达纯化及催化过程设计。
4、自行撰写开题报告与毕业论文。
2. 实验内容和要求
1、掌握人工PLP辅因子的基本合成方法2、掌握大肠杆菌重组细胞的培养、转氨酶诱导表达的基本实验操作3、掌握酶催化反应的基本操作。
3. 参考文献
1、Bea, H.-S.;Lee, S.-H.; Yun, H., Asymmetric synthesis of (R)-3-fluoroalanine from 3-fluoropyruvate using omega-transaminase. Biotechnology and Bioprocess Engineering 2011, 16 (2), 291-296.2、Bea, H.-S.;Seo, Y.-M.;Cha, M.-H.;Kim, B.-G.; Yun, H., Kinetic resolution of α-methylbenzylamine by recombinant Pichia pastoris expressing ω-transaminase. Biotechnology and Bioprocess Engineering 2010, 15 (3), 429-434.3、Cheng, Y.;Shan, Q.;Zhang, Y.;Quan, Z.;Zhang, K.; Wang, B., A highly efficient one-enzyme protocol using ω-transaminase and an amino donor enabling equilibrium displacement assisted by molecular oxygen. Organic Chemistry Frontiers 2018, 5 (10), 1633-1638.4、Kaulmann, U.;Smithies, K.;Smith, M. E. B.;Hailes, H. C.; Ward, J. M., Substrate spectrum of w-transaminase from Chromobacterium violaceum DSM30191 and its potential for biocatalysis. Enzyme and Microbial Technology 2007, 41 (5), 628-637.5、Mathew, S.; Yun, H., ω-Transaminases for the Production of Optically Pure Amines and Unnatural Amino Acids. ACS Catalysis 2012, 2 (6), 993-1001.6、Monti, D.;Forchin, M. C.;Crotti, M.;Parmeggiani, F.;Gatti, F. G.;Brenna, E.; Riva, S., Cascade Coupling of Ene-Reductases and w-Transaminases for the Stereoselective Synthesis of Diastereomerically Enriched Amines. ChemCatChem 2015, 7 (19), 3106-3109.7、Payer, S. E.;Schrittwieser, J. H.; Kroutil, W., Vicinal Diamines as Smart Cosubstrates in the TransaminaseCatalyzed Asymmetric Amination of Ketones. European Journal of Organic Chemistry 2017, 2017 (17), 2553-2559.8、Tian, K.; Li, Z., A simple biosystem for the high-yielding cascade conversion of racemic alcohols to enantiopure amines. Angew Chem Int Ed Engl 2020.9、Hollmann, F.;Arends, I. W. C. E.; Buehler, K., Biocatalytic Redox Reactions for Organic Synthesis: Nonconventional Regeneration Methods. ChemCatChem 2010, 2 (7), 762-782.10、Paul, C. E.;Arends, I. W. C. E.; Hollmann, F., Is Simpler Better? Synthetic Nicotinamide Cofactor Analogues for Redox Chemistry. ACS Catalysis 2014, 4 (3), 788-797.11、Wu, H.;Tian, C.;Song, X.;Liu, C.;Yang, D.; Jiang, Z., Methods for the regeneration of nicotinamide coenzymes. Green Chemistry 2013, 15 (7).12、Banik S D, Bankura A, Chandra A. A QM/MM simulation study of transamination reaction at the active site of aspartate aminotransferase: Free energy landscape and proton transfer pathways[J]. Journal of Computational Chemistry, 2020, 1-11.
4. 毕业设计（论文）计划
2020.12-2021.01 阅读文献，完成开题报告2021.03-2021.03 设计并合成5种人工PLP辅因子2021.04-2021.05 转氨酶菌株培养、转氨酶表达及酶催化实验。
2021.06-2021.06 整理数据，撰写毕业论文
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
