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1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，Elsevier、NCBI电子期刊，Springer Link全文电子期刊，pubmed等检索资源上查阅关于碘-131以及东亚钳蝎氯毒素靶向神经胶质瘤的相关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
1. 实验内容（1）学习东亚钳蝎氯毒素（BmK CT）修饰的功能化聚乙烯亚胺包裹金纳米颗粒的合成方法与鉴定手段；（2）通过紫外可见分光光度计测试纳米材料的体外稳定性；（3）通过MTT测试法测试纳米复合材料的细胞毒性；（4）通过流式细胞术、共聚焦显微镜测试细胞对纳米复合材料的靶向摄取能力；（5）考察制备的纳米复合材料的体外癌细胞成像功能；2．实验要求 熟练使用关键数据库查找相关文献解决实验问题，熟练掌握纳米复合材料的制备与表征技术，掌握MTT测试法测试纳米复合材料的细胞毒性的流程，熟练进行流式细胞术、共聚焦显微镜测试，熟练利用origin等绘图软件进行数据处理。
3. 参考文献
1) Lingzhou Zhao, Jingyi Zhu, Jinhua Zhao, et al. (2020). "A novel Buthus martensii Karsch chlorotoxin derivative for glioma SPECT imaging." New Journal of Chemistry, 44(35), 14947-14952.2) Cheng, Y. J., J. Y. Zhu, et al. (2016). "131I-labeled multifunctional dendrimers modified with BmK CT for targeted SPECT imaging and radiotherapy of gliomas." Nanomedicine 11(10): 1253-1266.3) Lingzhou Zhao, Yujie Li, Jinhua Zhao, et al. (2019). "Chlorotoxin peptide-functionalized polyethylenimine-entrapped gold nanoparticles for glioma SPECT/CT imaging and radionuclide therapy." Journal of Nanobiotechnology, 17:30.4) Xing, Y., J. Y. Zhu, et al. (2018). "SPECT/CT imaging of chemotherapy-induced tumor apoptosis using 99mTc-labeled dendrimer-entrapped gold nanoparticles." Drug Deliv. 25(1): 1384-1393.5) Zhao, L. Z., J. Y. Zhu, et al. (2015). "Chlorotoxin-conjugated multifunctional dendrimers labeled with radionuclide 131I for single photon emission computed tomography imaging and radiotherapy of gliomas." ACS Appl. Mater. Interfaces 7(35): 19798-19808.6) Jingyi Zhu, Lingzhou Zhao, Xiangyang Shi, et al. (2019). "99mTc-Labeled polyethylenimine-entrapped gold nanoparticles with pH-responsive charge conversion property for enhanced dual mode SPECT/CT imaging of cancer cells." Langmuir, 35(41): 13405-13412.7) Zhou, B., J. Yang, et al. (2016). "PEGylated polyethylenimine-entrapped gold nanoparticles modified with folic acid for targeted tumor CT imaging." Colloids and Surfaces B-Biointerfaces 140: 489-496.8) Zhu, J. Y., F. F. Fu, et al. (2015). "Dendrimer-entrapped gold nanoparticles modified with RGD peptide and alpha-tocopheryl succinate enable targeted theranostics of cancer cells." Colloids Surf., B 133: 36-42.9) Zhu, J. Y., L. Z. Zhao, et al. (2015). "Radionuclide 131I-labeled multifunctional dendrimers for targeted SPECT imaging and radiotherapy of tumors." Nanoscale 7(43): 18169-18178.10) Zhu, J. Y., L. F. Zheng, et al. (2014). "Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles." Biomaterials 35(26): 7635-7646.
4. 毕业设计（论文）计划
起讫日期 设计（论文）各阶段工作内容 备 注2021.1.2-2021.1.19 文献查询和资料阅读 2021.1.19-2021.1.23 研究方案制定和开题报告写作 2021.1.24-2021.3.10 实践内容的准备 2021.3.11-2021.5.10 实践 2021.5.10-2021.5.15 实践内容处理与结果分析 2021.5.16-2021.6.1 毕业论文书写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
