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1. 毕业设计（论文）的内容和要求
1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，Elsevier、NCBI电子期刊，Springer Link全文电子期刊，Web of Science等检索资源上查阅关于科罗索酸、内质网应激、JNK的相关文献。
2．文献阅读及综述 阅读与科罗索酸、内质网应激、JNK等相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。
3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
1. 实验内容（1）研究科罗索酸对前列腺癌细胞的存活率的影响；（2）研究科罗索酸对前列腺癌细胞凋亡的影响；（3）研究科罗索酸对JNK信号通路的影响。
2．实验要求熟练使用关键数据库查找相关文献解决实验问题，熟练掌握细胞培养技术，熟练掌握MTT实验，熟练掌握流式细胞仪检测细胞凋亡的实验方法，熟练掌握Western Blot检测蛋白表达的方法，熟练利用Origin等绘图软件进行数据处理。
3. 参考文献
1. Fallowfield L, Payne H, Jenkins V. Patient-reported outcomes in metastatic castration-resistant prostate cancer. Nat Rev Clin Oncol. 2016;13(10):64350.2. Litwin MS, Tan HJ. The diagnosis and treatment of prostate Cancer: a reviewJAMA. 2017;317(24):253242.3. Kallifatidis G, Hoy JJ, Lokeshwar BL. Bioactive natural products for hemoprevention and treatment of castration-resistant prostate cancer.Semin Cancer Biol. 2016;40-41:1609.4. Ritch CR, Cookson MS. Advances in the management of castration resistant prostate cancer. BMJ. 2016;355:i4405.5. Chevet E, Hetz C, Samali A. Endoplasmic reticulum stress-activated cell reprogramming in oncogenesis. Cancer Discov. 2015;5(6):58697.6. Ron D, Walter P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol. 2007;8(7):51929.7. Oakes SA, Papa FR. The role of endoplasmic reticulum stress in human pathology. Annu Rev Pathol. 2015;10:17394.8. Yamamoto K, Ichijo H, Korsmeyer SJ. BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normallyactivated at G(2)/M. Mol Cell Biol. 1999;19(12):846978.9. Yang Y, Liu L, Naik I, Braunstein Z, Zhong J, Ren B. Transcription factor C/EBPhomologous protein in health and diseases. Front Immunol. 2017;8:1612.10. Lamoureux F, Thomas C, Yin MJ, Fazli L, Zoubeidi A, Gleave ME. Suppression of heat shock protein 27 using OGX-427 induces endoplasmic reticulum stress and potentiates heat shock protein 90 inhibitors to delay castrateresistant prostate cancer. Eur Urol. 2014;66(1):14555.11. Wang M, Shim JS, Li RJ, Dang Y, He Q, Das M, et al. Identification of an old antibiotic clofoctol as a novel activator of unfolded protein response pathways and an inhibitor of prostate cancer. Br J Pharmacol. 2014;171(19):447889.12. Storm M, Sheng X, Arnoldussen YJ, Saatcioglu F. Prostate cancer and the unfolded protein response. Oncotarget. 2016;7(33):5405166.13. Firczuk M, Gabrysiak M, Barankiewicz J, Domagala A, Nowis D, Kujawa M, etal. GRP78-targeting subtilase cytotoxin sensitizes cancer cells to photodynamic therapy. Cell Death Dis. 2013;4:e741.14. Song JH, Kraft AS. Pim kinase inhibitors sensitize prostate cancer cells to apoptosis triggered by Bcl-2 family inhibitor ABT-737. Cancer Res. 2012;72(1):294303.15. Gafar AA, Draz HM, Goldberg AA, Bashandy MA, Bakry S, Khalifa MA, et al. Lithocholic acid induces endoplasmic reticulum stress, autophagy and mitochondrial dysfunction in human prostate cancer cells. PeerJ. 2016;4:e2445.16. Wang Q, Sun Q, Ma X, Rao Z, Li H. Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: spectroscopic and molecular modeling approaches. J Photochem Photobiol B. 2015;148:26876.17. Ahn KS, Hahm MS, Park EJ, Lee HK, Kim IH. Corosolic acid isolated from the fruit of Crataegus pinnatifida var. psilosa is a protein kinase C inhibitor as well as a cytotoxic agent. Planta Med. 1998;64(5):46870.18. Ku CY, Wang YR, Lin HY, Lu SC, Lin JY. Corosolic acid inhibits hepatocellular carcinoma cell migration by targeting the VEGFR2/Src/FAK pathway. PLoS One. 2015;10(5):e0126725.
4. 毕业设计（论文）计划
起讫日期 设计（论文）各阶段工作内容2021.1.7-2021.1.19 文献查询和资料阅读2021.1.19-2021.1.23 研究方案制定和开题报告撰写2021.1.24-2021.3.10 实验内容的准备2021.3.11-2021.5.10 实验内容处理2021.5.10-2021.5.15 实验结果分析2021.5.16-2021.6.1 毕业论文书写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
