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1. 毕业设计（论文）的内容和要求
1. 文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、Elsevier电子期刊、Springer Link全文电子期刊等检索资源，查阅关于细胞毒药物-多肽耦联的相关文献。
2. 文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。
3. 明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
实验内容：1. 确定含有目的蛋白的获取方案；2. 纯化获取的目的蛋白；3. 进行抗肿瘤的活性测试实验要求：1. 熟悉使用数据库进行文献检索2. 熟悉表达纯化的操作过程3. 熟悉抗肿瘤活性实验
3. 参考文献
1. Youn Y S , Shin M J , Chae S Y , et al. Biological and physicochemical evaluation of the conformational stability of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)[J]. Biotechnology Letters, 2007, 29(5):713-721.2. Guimaraes P P G , Gaglione S , Sewastianik T , et al. Nanoparticles for Immune Cytokine TRAIL-Based Cancer Therapy[J]. ACS Nano, 2018: 12(2): 912931.3. Yuan X , Gajan A , Chu Q , et al. Developing TRAIL/TRAIL death receptor-based cancer therapies[J]. Cancer and Metastasis Reviews, 20183: 7(4): 733748.4. Maiolo D , Pigliacelli C , Sanchez Moreno, Pola, et al. Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release[J]. ACS Nano, 2017: 11(9), 94139423.5. Valo H K , Paivi H. Laaksonen, Peltonen L J , et al. Multifunctional Hydrophobin: Toward Functional Coatings for Drug Nanoparticles[J]. ACS Nano, 2010, 4(3), 1750-1758.6. Zhao L , Xu H , Li Y , et al. Novel application of hydrophobin in medical science: a drug carrier for improving serum stability[J]. Scientific Reports, 2016, 6:26461.7. Sebastian S , Dominik S , Daniel R , et al. Fusion to Hydrophobin HFBI Improves the Catalytic Performance of a Cytochrome P450 System[J]. Frontiers in Bioengineering and Biotechnology, 2016, 4; 578. Wang X , Song D , Wang B , et al. A mutant of hydrophobin HGFI tuning the self-assembly behaviour and biosurfactant activity[J]. Applied Microbiology and Biotechnology, 2017, 101, 84198430.9. Macindoe I , Kwan A H , Ren Q , et al. Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS[J]. Proceedings of the National Academy of Sciences, 2012, 109(14): E804-E811.10. Berger B W , Sallada N D . Hydrophobins: multifunctional biosurfactants for interface engineering[J]. Journal of Biological Engineering, 2019, 13(1).
4. 毕业设计（论文）计划
1. 2021.1-2021.3：完成文献阅读2. 2020.3-2020.4：完成蛋白表达3. 2020.4-2020.5：纯化及初步探索抗肿瘤测试4. 2020.5-2020.6：抗肿瘤测试5. 2020.6：毕业论文撰写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
