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1. 毕业设计(论文)的内容和要求
1 文献阅读与归纳,深入了解课题背景2 重组腺苷酸环化酶的构建与诱导表达3掌握重组酶酶学性质分析方法4 独立完成毕业论文的撰写
2. 实验内容和要求
1 掌握大肠杆菌重组菌的诱导与表达的基本方法2 掌握酶活检测的基本操作3 掌握HPLC检测产物的方法
3. 参考文献
1 Ma C , Wang J , Wang X , et al. Efficient production of cyclic adenosine monophosphate from adenosine triphosphate by the N-terminal half of adenylate cyclase from Escherichia coli[J]. Chinese Journal of Chemical Engineering, 2020, 28(8).2 Cannella S E , Ntsogo Engun, Vronique Yvette, Davi M , et al. Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis.[J]. Scientific Reports, 2017, 7:42065.3 He Y , Li N , Chen Y , et al. Cloning, expression, and characterization of an adenylate cyclase from Arthrobacter sp. CGMCC 3584[J]. Applied Microbiology Biotechnology, 2012, 96(4):963-970.
4. 毕业设计(论文)计划
2020.12-2021.01 阅读文献,完成开题报告2021.03-2021.04 重组大肠杆菌的构建与重组酶的诱导表达2021.05-2021.05 重组酶酶学性质分析2021.06-2021.06 撰写毕业论文
以上是毕业论文任务书,课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。