1. 毕业设计（论文）的内容和要求
1.查阅检测脂肪酶活性方法的相关文献，了解荧光传感器的构建原理及对脂肪酶活性的检测；2.初步验证方案的可行性；3.进行实验方法的组装，包括实验条件的优化以及实验数据的处理等；4.归纳所得实验结论以及对实验进行展望。
2. 参考文献
[1] Bunch J S, Van Der Zande A M, Verbridge S S, et al. Electromechanical resonators from graphene sheets [J]. Science, 2007, 315(5811): 490-493. [2] Sun X, Liu Z, Welsher K, et al. Nano-graphene oxide for cellular imaging and drug delivery [J]. Nano research, 2008, 1(3): 203-212. [3] Dreyer D R, Park S, Bielawski C W, et al. The chemistry of graphene oxide [J]. Chemical Society Reviews, 2010, 39(1): 228-240. [4] Loh K P, Bao Q, Ang P K, et al. The chemistry of graphene [J]. Journal of Materials Chemistry, 2010, 20(12): 2277-2289. [5] Howes P D, Chandrawati R, Stevens M M. Colloidal nanoparticles as advanced biological sensors[J]. Science, 2014, 346(6205): 1247390. [6] Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, et al. Quantum dots versus organic dyes as fluorescent labels[J]. Nature methods, 2008, 5(9): 763-775. [7] Nozik A J, Beard M C, Luther J M, et al. Semiconductor quantum dots and quantum dot arrays and applications of multiple exciton generation to third-generation photovoltaic solar cells[J]. Chemical reviews, 2010, 110(11): 6873-6890. [8] Medintz I L, Uyeda H T, Goldman E R, et al. Quantum dot bioconjugates for imaging, labelling and sensing[J]. Nature materials, 2005, 4(6): 435-446. [9] Michalet X, Pinaud F F, Bentolila L A, et al. Quantum dots for live cells, in vivo imaging, and diagnostics[J]. science, 2005, 307(5709): 538-544. [10] Wu P, Zhao T, Wang S, et al. Semicondutor quantum dots-based metal ion probes[J]. Nanoscale, 2014, 6(1): 43-64. [11] Lou Y, Zhao Y, Chen J, et al. Metal ions optical sensing by semiconductor quantum dots[J]. Journal of Materials Chemistry C, 2014, 2(4): 595-613. [12] Zhang Y, Zhang H, Ma M, et al. The influence of ligands on the preparation and optical properties of water-soluble CdTe quantum dots[J]. Applied Surface Science, 2009, 255(9): 4747-4753. [13] Mohamed Ali E, Zheng Y, Yu H, et al. Ultrasensitive Pb2 detection by glutathione-capped quantum dots[J]. Analytical chemistry, 2007, 79(24): 9452-9458. [14] Wang X, Lv Y, Hou X. A potential visual fluorescence probe for ultratracearsenic (III) detection by using glutathione-capped CdTe quantum dots[J]. Talanta, 2011, 84(2): 382-386. [15] Ke J, Li X, Shi Y, et al. A facile and highly sensitive probe for Hg (II) based on metal-induced aggregation of ZnSe/ZnS quantum dots[J]. Nanoscale, 2012, 4(16): 4996-5001. [16] Zhu X, Zhao Z, Chi X, et al. Facile, sensitive, and ratiometric detection of mercuric ions using GSH-capped semiconductor quantum dots[J]. Analyst, 2013, 138(11): 3230-3237. [17] Wang H, Li Y, Fei X, et al. Synthesis and characterization of multifunctional CdTe/Fe2O3@ SiO2 core/shell nanosensors for Hg2 ions detection[J]. New Journal of Chemistry, 2010, 34(12): 2996-3003. [18] Ge S, Zhang C, Zhu Y, et al. BSA activated CdTe quantum dot nanosensor for antimony ion detection[J]. Analyst, 2010, 135(1): 111-115. [19] Sung T W, Lo Y L. Highly sensitive and selective sensor based on silica-coated CdSe/ZnS nanoparticles for Cu2 ion detection[J]. Sensors and Actuators B: Chemical, 2012, 165(1): 119-125. [20] Duan J, Jiang X, Ni S, et al. Facile synthesis of N-acetyl-l-cysteine capped ZnS quantum dots as an eco-friendly fluorescence sensor for Hg2 [J]. Talanta, 2011, 85(4): 1738-1743. [21] Chen Y, Rosenzweig Z. Luminescent CdS quantum dots as selective ion probes[J]. Analytical Chemistry, 2002, 74(19): 5132-5138. [22] Spanhel L, Haase M, Weller H, et al. Photochemistry of colloidal semiconductors. 20. Surface modification and stability of strong luminescing CdS particles[J]. Journal of the American Chemical Society, 1987, 109(19): 5649-5655. [23] Chen L, Zhang J, Luo Y, et al. Effect of Zn2 and Mn2 introduction on the luminescent properties of colloidal ZnS: Mn2 nanoparticles[J]. Applied physics letters, 2004, 84(1): 112-114. [24] Xu H, Miao R, Fang Z, et al. Quantum dot-based turn-on fluorescent probe for detection of zinc and cadmium ions in aqueous media[J]. Analytica chimica acta, 2011, 687(1): 82-88.[25] Chen J L, Zhu C Q. Functionalized cadmium sulfide quantum dots as fluorescence probe for silver ion determination[J]. Analytica Chimica Acta, 2005, 546(2): 147-153. [26] Sahu A, Kang M S, Kompch A, et al. Electronic impurity doping in CdSe nanocrystals[J]. Nano letters, 2012, 12(5): 2587-2594. [27] Xia Y S, Cao C, Zhu C Q. Two distinct photoluminescence responses of CdTe quantum dots to Ag (I)[J]. Journal of luminescence, 2008, 128(1): 166-172. [28] Ruedas-Rama M J, Hall E A H. Azamacrocycle activated quantum dot for zinc ion detection[J]. Analytical chemistry, 2008, 80(21): 8260-8268. [29] Mayilo S, Hilhorst J, Susha A S, et al. Energy transfer in solution-based clusters of CdTe nanocrystals electrostatically bound by calcium ions[J]. The Journal of Physical Chemistry C, 2008, 112(37): 14589-14594. [30] Sapsford K E, Berti L, Medintz I L. Materials for fluorescence resonance energy transfer analysis: beyond traditional donoracceptor combinations[J]. Angewandte Chemie International Edition, 2006, 45(28): 4562-4589.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
