1. 毕业设计（论文）的内容和要求
本课题以非芽孢菌施氏假单胞菌为研究对象，构建微胶囊制剂，旨在提高菌剂常温储藏条件下的稳定性和存活率。
要求完成菌剂微胶囊的制备及工艺优化及保质期初步评价工作。
2. 参考文献
[1] Zhao Z, Hamdan N, Shen L, Nan HQ, Almajed A, Kavazanjian E, He XM. Biomimetic hydrogel composites for soil stabilization and contaminant mitigation[J]. Environ. Sci. Technol, 2016, 50: 12401-12410.[2] Zvinavashe AT, Lim E, Sun H, Marelli B. A bioinspired approach to engineer seed microenvironment to boost germination and mitigate soil salinity[J]. PNAS, 2019, 116(51): 25555-25561.[3] Gebbers R, Adamchuk VI. Precision agriculture and food security[J]. Science, 2010, 327(5967): 828-831.[4] Bulgarelli D, Schlaeppi K, Spaepen S, Ver Loren van Themaat E, Schulze-Lefert P. Structure and functions of the bacterial microbiota of plants[J]. Annu. Rev. Plant. Biol, 64, 807838 (2013).[5] Kaminsky LM, Trexler RV, Malik RJ, Hockett KL, Bell TH. The inherent conflicts in developing soil microbial inoculants[J]. Trends. Biotechnol, 2019, 37(2): 140-151.[6] Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture[J]. World J. Microbiol. Biotechnol, 2012, 28(4): 1327-1350. [7] Abinandan S, Subashchandrabose SR, Venkateswarlu K, Megharaj M. Soil microalgae and cyanobacteria: the biotechnological potential in the maintenance of soil fertility and health[J]. Crit. Rev. Biotechnol, 2019, 39(8): 981-998.[8] Nadeem SM, Ahmad M, Zahir ZA, Javaid A, Ashraf M. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments[J]. Biotechnol. Adv, 2014, 32(2): 429-448. [9] Yu Y, Zhang W, Chen GH, Gao YC, Wang JN. Preparation of petroleum-degrading bacterial agent and its application in remediation of contaminated soil in Shengli Oil Field, China[J]. Environ. Sci. Pollut. R, 2014, 21: 7929-7937. [10] Berninger T, Lpez G, Bejarano A, Preininger C, Sessitsch A. Maintenance and assessment of cell viability in formulation of non-sporulating bacterial inoculants[J]. Microb. Biotechnol, 2018, 11(2): 277-301.[11] 何艳慧. 微胶囊化解盐促生菌株 SRPG-396 与棉花互作特性研究[D]. 石河子大学, 2016.[12] Melin P, Hkansson S, Schnrer J. Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121[J]. Appl Microbiol Biotechnol, 2007, 73: 1008-1016.[13] Hama R, OCallaghanb M, Geurtsa R, Ridgwayc HJ, Ballardd R, Nobleb A, Macarae G, Wakelinb SA. Soil moisture decit selects for desiccation tolerant Rhizobium leguminosarum bv. trifolii[J]. Appl Soil Ecol , 2016, 108: 371-380.
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