全文总字数：3009字
1. 毕业设计（论文）的内容和要求
1、毕业论文内容生物乳化剂是由各种各样的微生物产生的高分子量生物聚合物，可以有效地形成和稳定水包油乳液[1]。
它们是由多糖、脂多糖、脂蛋白和蛋白质等组成的两亲复合物[1-3]。
与表面活性剂相类似，生物乳化剂也能够提高难溶底物的生物利用度，从而促进难溶底物的生物降解[4]。
2. 实验内容和要求
1、实验内容1) 培养基的配制、灭菌以及AXE、E26蛋白的发酵培养。
2) AXE、E26蛋白的提取及纯化。
3) AXE、E26蛋白在MEOR应用中的评价（表界面张力、乳化性、降黏性等）。
3. 参考文献
1. Uzoigwe C, Burgess JG, Ennis CJ, Rahman PK: Bioemulsifiers are not biosurfactants and require different screening approaches. Front Microbiol. 2015;6:245.2. Dastgheib SMM, Amoozegar MA, Elahi E, Asad S, Banat IM: Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnol Lett. 2008;30:263-270.3. Toren A, Navonvenezia S, Ron EZ, Rosenberg E: Emulsifying activities of purified Alasan proteins from Acinetobacter radioresistens KA53. Appl Environ Microb. 2001;67:1102.4. Wang Y, Wang C, Ren HJ, Jia BL, Zhang LY: Effectiveness of recombinant protein AlnA in enhancing the extractability of polychlorinated biphenyls from contaminated soils. J Hazard Mater. 2014;279:67-74.5. Deleu M, Lorent J, Lins L, Brasseur R, Braun N, El Kirat K, Nylander T, Dufrene YF, Mingeot-Leclercq MP: Effects of surfactin on membrane models displaying lipid phase separation. BBA-Biomembranes. 2013;1828:801-815.6. Laha S, Luthy RG: Inhibition of phenanthrene mineralization by nonionic surfactants in soil-water systems. Environ Sci Technol. 1991;25:1920-1930.7. Lamichhane S, Krishna KCB, Sarukkalige R: Surfactant-enhanced remediation of polycyclic aromatic hydrocarbons: A review. J Environ Manage. 2017;199:46-61.8.Tao WY, Lin JZ, Wang WD, Huang H, Li S: Designer bioemulsifers based on combinations of diferent polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034. Microb Cell Fact. 2019; 18:173.9.Cao GS, Du T, Bai YJ, Yang TY, Zuo JZ: Effects of surfactant molecular structure on the stability of water in oil emulsion. J Petrol Sci Eng. 2020; 196:107695.
4. 毕业设计（论文）计划
12.30~1.15 文献查阅及资料研读1.16~2.25 确定实验方案及开题报告写作2.26~3.30 AXE、E26蛋白的培养与提取3.31~5.15 AXE、E26蛋白及相应多糖体系在MEOR中的应用评价5.16~5.31 毕业论文撰写
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