1. 毕业设计（论文）的内容和要求
一、论文内容：L-赖氨酸脱羧酶（Lysine decarboxylase）存在于很多自然界的微生物中，如蜂房哈夫尼菌、大肠杆菌、产酸克雷伯菌等[1]，甚至一些高等植物中也含有L-赖氨酸脱羧酶[2]。
其可以以L-赖氨酸为底物，催化脱羧作用可以生产戊二胺[3]。
而戊二胺是一种重要的平台化合物,可以合成高附加值、性能优异的新型聚氨酯、新型聚酰胺等材料[4,5,6]。
2. 参考文献
[1] Young Hoon Oh, Kyoung-Hee Kang, Mi Jeong Kwon, et al. Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of L-lysine into cadaverine[J]. J Ind Microbiol Biotechnol, 2015, (42): 1481-1491.[2] Sabo D L, Boeler E A, Byers E, et al. Purification and physical properties of inducible Escherichia coli lysine decarboxylase[J]. Biochemistry,1974,13(4): 662-670.[3] Nishi Kiyohiko, Endo Shuichi, Mori Yukiko, et al. Method for Producing cadaverine diearboxylate[P]. UniteStatesPetent:20050003497, 2005-00-03. [3] [4] Sanders J, Scott E, Weusthuis R, et al. Bio-refinery as the bio-inspired process to bulk chemicals [J]. Macromolecular Bioscience, 2007, 7(2): 105-117.[5] Eltahir Y A, Saeed H a M, Chen Y, et al. Effect of hot drawing on the structure and properties of novel polyamide 5,6 fibers [J]. Textile Research Journal, 2014, 84(16): 1700-7.[6] 于维才. 尼龙56的物理性能及可纺性探析[J]. 聚酯工业, 2014, 27(1): 38-39.[7] Kind S , Neubauer S , Becker J , et al. From zero to hero Production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum[J]. Metabolic Engineering, 2014, 25:113-123.[8] Kim H J, Kim Y H, Shin J H, et al. Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration[J]. 2015, 25(7):1108.[9] Kou F , Zhao J , Liu J , et al. Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH[J]. Journal of Molecular Catalysis B: Enzymatic, 2016:S1381117716302697.
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