1. 毕业设计（论文）的内容和要求
（1）论文内容采用基因工程手段，构建质粒pRSFDuet-UGT，并导入大肠杆菌。
以大肠杆菌为宿主诱导表达产酶，对植物来源的糖基转移酶进行高效表达，并对重组酶进行纯化。
（2）论文要求 1．文献查阅。
2. 参考文献
[1] MOONS N, de BORGGRAEVE W, DEHAEN W. Stevioside and steviol as starting materials in organic synthesis[J]. Current Organic Chemistry, 2012, 16(17): 1986-1995. [2] Bechthold G B E P. Identification of a sugar fexible glycosyltransferase from Streptomyces olivaceus, the producer of the antitumor polyketide elloramycin [J]. Chemistry Biology, 2001, 8: 253~263. [3] Cantarel B L, Coutinho P M, Rancurel C, et al. The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics [J]. Nucleic Acids Research, 2009, 37(Database): D233~D238. [4] Gantt R W, Peltier-Pain P, Thorson J S. Enzymatic methods for glycol (diversification/randomization) of drugs and small molecules [J]. Nat Prod Rep, 2011, 28(11):1811~1853.[5] Joe Ross Y L E L. Higher plant glycosyltransferases [J]. Genome Biology, 2001, 2(2):3001~3004. [6] Paquette S M B B S. On the origin of family 1 plant glycosyltransferases [J]. Phytochemistry, 2003, 62(3): 399~413.[7] RICHMAN A S, GIJZEN M, STARRATT A N, et al. Diterpene synthesis in Stevia rebaudiana: recruitment and up -regulation of key enzymes from the gibberellin biosynthetic pathway[J]. Plant Journal for Cell Molecular Biology, 1999, 19(4): 411-421 [8] XU Zhongwei, LI Yuqiang, WANG Yonghua, et al. Production of fructofuranosidase by Arthrobacter sp. and its application in the modification of stevioside and rebaudioside A[J]. Food Technology and Biotechnology, 2009, 47(2): 137-143. [9] NIKIFOROV A I, RIHNER M O, EAPEN A K, et al. Metabolism and toxicity studies supporting the safety of rebaudioside D[J]. International Journal of Toxicology, 2013, 32(4): 261-273. [10] PURKAYASTHA S, PUGH G, Jr, LYNCH B, et al. In vitro metabolism of rebaudioside B, D, and M under anaerobic conditions: comparison with rebaudioside A[J]. Regulatory Toxicology and Pharmacology, 2014, 68(2): 259-268. [11] De Bruyn F, Maertens J, Beauprez J, et al. Biotechnological advances in UDP-sugar based glycosylation of small molecules [J]. Biotechnology Advances, 2015, 33(2):288~302.[12] Yang Y H, HuangSZ, Han Y L,et al. Base substitutionmutations in uridinediphosphate-dependent glycosyltransferase 76G1 gene of Stevia rebaudiana causes the low levels of rebaudioside A: mutations in UGT76G1, a keygene of steviol glycosides synthesis[J]. Plant Physiologyand Biochemistry : PPB / Societe Francaise de Physiologie Vegetale, 2014, 80:220 - 225.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
