1. 毕业设计（论文）的内容和要求
本论文主要以S-ω-转氨酶的保守序列进行基因挖掘，通过重组质粒，进行酶学表征。
主要从以下几点进行阐述：1.序列筛选比对分析2.质粒构建3.酶学性质表征
2. 参考文献
1. Jiang, J.; Chen, X.; Feng, J.; Wu, Q.; Zhu, D. Substrate profile of an ω-transaminase from Burkholderia vietnamiensis and its potential for the production of optically pure amines and unnatural amino acids[J]. Journal of Molecular Catalysis B Enzymatic, 2014, 100:32-39.2. Kaulmann, U.; Smithies, K.; Smith, M.E.B.; Hailes, H.C. Ward, J.M. Substrate spectrum of ω-transaminase from Chromobacterium violaceum DSM30191 and its potential for biocatalysis[J]. Enzyme Wohlgemuth, R.; Ward, J.M. Synthesis of pyridoxamine 5′-phosphate using an MBA:pyruvate transaminase as biocatalyst[J]. Journal of Molecular Catalysis B Enzymatic, 2009, 59(4):279-285.4. Zhu, W.; Li, Y.; Jia, H.; Wei, P.; Zhou, H. Expression, purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari[J]. Biotechnology Letters, 2016, 38(5):855-861.5. Ferrandi, EE.; Previdi, A.; Bassanini, I.; Riva, S.; Peng, X. Monti D. Novel thermostable amine transferases from hot spring metagenomes[J]. Applied Microbiology and Biotechnology, 2017, 101(5): 1-17.6. Mathew, S.; Nadarajan, SP.; Chung, T.; Park, HH.; Yun, H. Biochemical characterization of thermostableω-transaminase from Sphaerobacter thermophilus and its application for producing aromatic β- and γ-amino acids[J]. Enzyme Microbial Technology, 2016, 87-88: 52-60.
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