1. 毕业设计（论文）的内容和要求
课题简介持续性内切酶（Processive endoglucanase）作为一种特殊的双功能纤维素水解酶，不但具有内切葡聚糖酶的水解特性，还表现出外切葡聚糖酶持续性水解的能力，为减少纤维素降解的酶成本提供了可能。
前期实验室通过蛋白质工程改造获得了酶活力显著提高持续性内切酶突变体D70Q/S235W。
然而绝大多数酶类是具有催化活性的蛋白质，构成酶分子的氨基酸残基种类和序列决定了酶的高级结构及其不同环境条件下的催化性能。
2. 参考文献
1.Wu B , Zheng S , Pedroso M M , et al. Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates[J]. Biotechnology for Biofuels, 2018, 11(1):20.2.Cao L , Li S , Huang X , et al. Enhancing the thermostability of highly active and glucose-tolerant β-glucosidase Ks5A7 by directed evolution for good performance on three properties[J]. Journal of Agricultural and Food Chemistry, 2018.3.Ibrahim, Torktaz, Ali, et al. Rational engineering of Cel5E from Clostridium thermocellum to improve its thermal stability and catalytic activity.[J]. Applied microbiology and biotechnology, 2018.4.Lin L , Fu C , Huang W . Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR[J]. Enzyme and Microbial Technology, 2016, 86:52-58.5.Ma M , Okuyama M , Sato M , et al. Effects of mutation of Asn694 in Aspergillus niger α-glucosidase on hydrolysis and transglucosylation[J]. Applied Microbiology and Biotechnology, 2017, 101(16):1-10.6.Zheng F , Tu T , Wang X , et al. Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6[J]. Biotechnology for Biofuels, 2018, 11(1):76.7.Jing-Jing, Chen, Xiao, et al. Site-Directed Mutagenesis of a β-Glycoside Hydrolase from Lentinula Edodes.[J]. Molecules (Basel, Switzerland), 2018.8.张瑞萍. 纤维素酶的滤纸酶活和CMC酶活的测定[J]. 印染助剂，2002(05)：51-53.9.陶敏，王振兴，陈晓艺. 持续性内切纤维素酶高效催化的研究进展[J]. 微生物学杂志，2019，39 (05)：98-104.10.张小梅，李单单，王禄山，等. 纤维素酶家族及其催化结构域分子改造的新进展[J]. 生物工程学报，2013，29(04)：422-433.11.Akbarzadeh A，Pourzardosht N，Dehnavi E，et al. Disulfide bonds elimination of endoglucanase II from Trichoderma reesei by site-directed mutagenesis to improve enzyme activity and thermal stability: An experimental and theoretical approach[J]. International Journal of Biological Macromolecules，2018，120：1572-1580.12.Taylor L E，Knott B C，Baker J O，et al. Engineering enhanced cellobiohydrolase activity[J]. Nature Communications，2018，9(1).
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
