全文总字数：3342字
1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-CoA含量对菌体产油脂和神经酸情况的影响，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
本课题在实验室现有的对解脂耶氏酵母代谢研究的基础上，结合国内外解脂耶氏酵母产油代谢途径优化的代谢工程和合成生物学方面的工具，研究解脂耶氏酵母产油途径，以调节脂肪酸比例生产神经酸。
实验内容包括：1. 熟悉酵母基因工程、分子生物学相关知识及实验操作； 2. 构建相关基因敲除质粒及解脂耶氏酵母重组菌株； 3. 气相色谱测定解脂耶氏酵母重组菌株的油脂合成产量及脂肪酸途径。
3. 参考文献
1)Tanaka K, Shimizu T, Ohtsuka Y, Yamashiro Y, Oshida K (2007) Early dietary treatments with Lorenzos oil and docosahexaenoic acid for neurological development in a case with Zellweger syndrome. Brain Dev 29:586598 2)2. Amminger GP, Schafer MR, Klier CM, Slavik JM, Holzer I, Holub M, Goldstone S, Whitford TJ, McGorry PD, Berk M (2012) Decreased nervonic acid levels in erythrocyte membranes pre- dict psychosis in help-seeking ultra-high-risk individuals. Mol Psychiatry 17:11501152 3)3. Sun X, Pang H, Li M, Peng B, Guo H, Yan Q, Hang Y (2013) Evolutionary pattern of the FAE 1 gene in Brassicaceae and its correlation with the erucic acid trait. PLoS One 8(12):e83535 4)James DW, Lim E, Keller J, Plooy I, Ralston E, Dooner HK (1995) Directed tagging of the Arabidopsis fatty acid elongation 1 (FAE1) gene with the maize transposon activator. Plant Cell 7:309319 5)Taylor DC, Francis T, Guo S, Brost JM, Katavic V, Mietkiewska E, Giblin EM, Lozinsky S, Hoffman T (2009) Molecular cloning and charac- terization of a KCS gene from Cardamine graeca and its heterologous expression in Brassica oilseeds to engineer high nervonic acid oils for potential medical and industrial use. Plant Biotechnol J 7:925938 6)Huai D, Zhang Y, Zhang C, Cahoon EB, Zhou Y (2015) Combinatorial effects of fatty acid Elongase enzymes on Nervonic acid production in Camelina sativa. PLoS One 10:e0131755 7)Marillia EF, Francis T, Falk KC, Smith M, Taylor DC (2014) Palliser's promise: Brassica carinata, an emerging western Canadian crop for delivery of new bio-industrial oil feedstocks. Biocatal Agric Biotechnol 3:6574 8)Guo Y, Mietkiewska E, Francis T, Katavic V, Brost JM, Giblin M, Barton DL, Taylor DC (2009) Increase in nervonic acid content in trans- formed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene. Plant Mol Biol 69:565675 9)Millar AA, Kunst L (1997) Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J 12:101111 10)Han J, Luhs W, Sonntag K, Zahringer U, Borchardt DS, Wolter FP, Heinz E, Frentzen M (2001) Functional characterization of Beta-Ketoacyl- CoA synthase genes from Brassica napus L. Plant Mol Biol 46: 229239 11)Mietkiewska E, Giblin EM, Wang S, Barton DL, Dirpaul J, Brost JN, Katavic V, Taylor DC (2004) Seed-specific heterologous expression of a Nasturtium FAE gene in Arabidopsis results in a dramatic in- crease in the proportion of erucic acid. Plant Physiol 136:26652675
4. 毕业设计（论文）计划
2020.12.1-2020.12.30 确定毕业设计的课题及相关资料的查找2020.12.31-2020.1.28 实验方案的设计2020.1.29-2020.2.28 实验材料的准备2020.2.28-2020.5.14 准备分子实验2020.5.15-2020.5.19 实验数据处理与结果分析2020.5.20-2020.6.3 毕业论文书写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
