全文总字数：3781字
1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解重组解脂耶氏酵母生产(-)-α-红没药醇的代谢过程与发酵工艺优化方法，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
本课题在实验室现有的产(-)-α-红没药醇重组菌株的基础上，结合国内外报道的解脂耶氏酵母产萜类化合物的发酵条件，研究解脂耶氏酵母产萜类途径，以调节发酵条件优化(-)-α-红没药醇生产。
实验内容包括：1. 熟悉酵母发酵工艺相关知识及实验操作； 2.利用分批补料发酵优化(-)-α-红没药醇生产； 3. 使用气相色谱测定解脂耶氏酵母重组菌株的(-)-α-红没药醇合成产量。
3. 参考文献
1） Han, G. H., S. K. Kim, P. K. Yoon, Y. Kang, B. S. Kim, Y. Fu, B. H. Sung, H. C. Jung, D. H. Lee, S. W. Kim and S. G. Lee (2016). "Fermentative production and direct extraction of (-)-α-bisabolol in metabolically engineered Escherichia coli." Microb Cell Fact 15(1): 185.2) Ma, Y., W. Li, J. Mai, J. Wang, Y. Wei, R. Ledesma-Amaro and X.-J. Ji (2020). "Engineering Yarrowia lipolytica for sustainable production of the chamomile sesquiterpene ()-α-bisabolol." Green Chemistry.3) Bouvier, F., Rahier, A., Camara, B. 2005. Biogenesis, molecular regulation and function of plant isoprenoids. Progress in lipid research, 44(6), 357-429.4) Kildegaard, K.R., Adiego-Perez, B., Domenech Belda, D., Khangura, J.K., Holkenbrink, C., Borodina, I. 2017. Engineering of Yarrowia lipolytica for production of astaxanthin. Synth Syst Biotechnol, 2(4), 287-294.5) Li, J., Fang, X., Zhao, Q., Ruan, J., Yang, C., Wang, L., Miller, D., Faraldos, J., Allemann, R., Chen, X., Zhang, P. 2013. Rational engineering of plasticity residues of sesquiterpene synthases from Artemisia annua: product specificity and catalytic efficiency. Biochem. J., 451(3), 417-26.6) Liao, P., Hemmerlin, A., Bach, T.J., Chye, M.-L. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology advances, 34(5), 697-713.7) Nielsen, J., Keasling, Jay D. 2016. Engineering Cellular Metabolism. Cell, 164(6), 1185-1197.8) Roberts, S.C. 2007. Production and engineering of terpenoids in plant cell culture. Nature chemical biology, 3(7), 387-395.9) Rohdich, F., Hecht, S., Grtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A., Eisenreich, W. 2002. Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein. Proceedings of the National Academy of Sciences, 99(3), 1158-1163.10) Son, Y.J., Kwon, M., Ro, D.K., Kim, S.U. 2014. Enantioselective microbial synthesis of the indigenous natural product (-)-alpha-bisabolol by a sesquiterpene synthase from chamomile (Matricaria recutita). Biochem J, 463(2), 239-48.11) Yang, X., Nambou, K., Wei, L., Hua, Q. 2016. Heterologous production of α-farnesene in metabolically engineered strains of Yarrowia lipolytica. Bioresour Technol, 216(2016), 1040-8.12) Madzak C. Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations. Mol Biotechnol. 2018. 60(8), 621-635.13) Lazar Z, Liu N, Stephanopoulos G. Holistic Approaches in Lipid Production by Yarrowia lipolytica. Trends in Biotechnology. 2018;36(11):1157-70.14) Darvishi F, Ariana M, Marella ER, Borodina I. Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals. Appl Microbiol Biotechnol. 2018;102(14):5925-38.15) Abdel-Mawgoud AM, Markham KA, Palmer CM, Liu N, Stephanopoulos G, Alper HS. Metabolic engineering in the host Yarrowia lipolytica. Metabolic Engineering. 2018.
4. 毕业设计（论文）计划
2020.12.1-2020.12.30 确定毕业设计的课题及相关资料的查找2020.12.31-2020.1.28 实验方案的设计2020.1.29-2020.2.28 实验材料的准备2020.2.28-2020.5.14 准备发酵实验2020.5.15-2020.5.19 实验数据处理与结果分析2020.5.20-2020.6.3毕业论文书写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
