1. 1. 毕业设计（论文）的内容、要求、设计方案、规划等
、知识和技能要求
⑴、具有扎实的基因工程知识基础，熟知PCR原理。
⑵、能够准确、熟练地掌握相关微生物学、基因操作实技术，如：PCR技术，蛋白异源表达方法，高效液相色谱的使用等。
2. 参考文献（不低于12篇）
其他指导教师：无其他指导教师 |
1. Furuya, Toshiki; Miura, Misa; Kino, Kuniki （2014） A Coenzyme-Independent Decarboxylase/Oxygenase Cascade for the Efficient Synthesis of Vanillin，CHEMBIOCHEM，15: 2248-2254 2. Wangrangsimagul, Nuttawat; Klinsakul, Kunticha; Vangnai, Alisa S. （2012）Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY，93 ：555-563 3. Jung DH, Choi W, Choi KY, Jung E, Yun H, Kazlauskas RJ, Kim BG (2013) Bioconversion ofp-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system. Appl Microbiol Biotechnol 97:15011511 4. Li XM, Yang JK, Li X, Gu W, Huang JW, Zhang KQ (2008) The metabolism of ferulic acid via 4-vinylguaiacol to vanillin by Enterobacter sp. Px6-4 isolated from vanilla root. ProcessBiochem 43:11321137 5. Weickert, M.J., Doherty, D.H., Best, E.A., Olins, P.O., 1996. Optimization of heterologous protein production in Escherichia coli. Curr. Opin. Biotechnol. 7, 494499 6. Ryu, Ji-Young; Seo, Jiyoung; Unno, Tatsuya （2010）Isoeugenol monooxygenase and its putative regulatory gene are located in the eugenol metabolic gene cluster in Pseudomonas nitroreducens Jin1 ARCHIVES OF MICROBIOLOGY192: 201-209 7. Yamada, Mamoru; Okada, Yukiyoshi; Yoshida, Toyokazu （2008）Vanillin production using Escherichia coli cells over-expressing isoeugenol monooxygenase of Pseudomonas putida，BIOTECHNOLOGY LETTERS30: 665-670 8. Marasco, Erinn K.; Schmidt-Dannert, Claudia （2008）Identification of bacterial carotenoid cleavage dioxygenase homologues that cleave the interphenyl alpha,beta double bond of stilbene derivatives via a monooxygenase reaction CHEMBIOCHEM9: 1450-1461 9. 赵金星，张梁，顾正华，丁重阳，石贵阳（201 3）重组大肠杆菌表达铜绿假单胞菌溶血性磷脂酶 C，微生物学报 53 ( 3 ) : 259－268 10. 向 阳, 王和勇, 雷 桅 , 孙 敏 （2008）环加氧酶 2 基因的大肠杆菌表达与纯化，西 南 大 学 学 报 30:121-125. 11.Gu W, Li X, Huang J, Duan Y, Meng Z, Zhang KQ, Yang J. Cloning, sequencing, and overexpression in Escherichia of the Enterobacter sp. Px6-4 gene for ferulic acid decarboxylase. Appl Microbiol Biotechnol, 2011, 89(6):1797-1805 12. Cavin J-F, Dartois V, Divis C. Gene cloning, transcriptional analysis, purification, and characterization of phenolic aicd decarboxylase from Bacillus subtilis. Appl Environ Microbiol, 1998, 64:1466-1471 |
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