1. 毕业设计(论文)的内容、要求、设计方案、规划等
前言应包括研究背景意义、国内外研究现状和课题的立体依据。
需要广泛查阅与课题有关的文献,充分掌握一手、二手资料,运用恰当充分。
确定该论文研究的理论基础或依据。
2. 参考文献(不低于12篇)
1. Zhou, X. and J.C. Wu, Heterologous expression and characterization of Bacillus coagulans L-arabinose isomerase. World J Microbiol Biotechnol, 2012. 28(5): p. 2205-12.2. Malakar, P. and K.V. Venkatesh, Effect of substrate and IPTG concentrations on the burden to growth of Escherichia coli on glycerol due to the expression of Lac proteins. Appl Microbiol Biotechnol, 2012. 93(6): p. 2543-9.3. Olaofe, O.A., et al., Improving the production of a thermostable amidase through optimising IPTG induction in a highly dense culture of recombinant Escherichia coli. Biochemical Engineering Journal, 2010. 52(1): p. 19-24.4. Dische Z, Borenfreund E.A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192:5835874. 程丽芳. 解纤维热酸菌L-阿拉伯糖异构酶的克隆表达、性质研究及分子改造.江南大学.20105. Rhimi M, Bejar SCloning, purification and biochemical characterization of metallic-ions independent and thermoactive L-arabinose isomerase from the Bacillus stearothermophilus US100 strain. Biochim Biophys Acta ,2010.1760:1911996. Rhimi M, Juy M, Aghajari N, Haser R, Bejar S Probing the essential catalytic residues and substrate affinity in the thermoactive Bacillus stearothermophilus US100 L-arabinose isomerase by site-directed mutagenesis. J Bacteriol ,2007.189:355635637. Boudebbouze, Samira;Maguin, Emmanuelle;Rhimi, MoezBacterial L-Arabinose Isomerases: Industrial Application for D-Tagatose Production.Recent Patents on DNA Gene Sequences,2011. 3(5): 194-2018. 程丽芳,沐万孟,张涛等.微生物来源的L-阿拉伯糖异构酶的研究进展及应用前景[J].微生物学通报,2008,35(10):1626-1632.9. 翁玮慧,张华,江波等.L-阿拉伯糖异构酶粗酶性质及其生物合成塔格糖的研究[J].食品工业科技,2006,27(1):158-160,162.10.程丽芳,沐万孟,张涛等.Bacillus Stearothermophilus IAM 11001 L-阿拉伯糖异构酶在大肠杆菌中的表达、纯化及活性研究[J].中国生物工程杂志,2008,28(9):52-55.
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