1. 毕业设计（论文）的内容和要求
1. 完成AlyPL17序列生物信息学分析2. 完成AlyPL17的酶学性质表征及降解模式分析3. 利用同源建模及分子对接阐明酶与底物选择性机制4. 完成南京工业大学对于毕业论文的要求
2. 参考文献
1. Zhu B, Yin H. Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications[J]. Bioengineered, 2015, 6(3):125-131.2. Yu Z, Zhu B, Wang W, et al. Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp[J]. International Journal of Biological Macromolecules, 2018, 112:937.3. Huang G, Wen S, Liao S, et al. Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2[J]. Biotechnology Letters, 2019, 41(1).4. Jagtap, S. S, Hehemann, J.-H, Polz, M. F, et al. Comparative Biochemical Characterization of Three Exolytic Oligoalginate Lyases from Vibrio splendidus Reveals Complementary Substrate Scope, Temperature, and pH Adaptations[J]. Applied Environmental Microbiology, 80(14):4207-4214.5. Kim H T, Chung J H, Wang D, et al. Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans2-40[J]. Applied Microbiology Biotechnology, 2012, 93(5):2233-2239.6. Park H H, Kam N, Lee E Y, et al. Cloning and Characterization of a Novel Oligoalginate Lyase from a Newly Isolated BacteriumSphingomonassp. MJ-3[J]. Marine Biotechnology, 2012, 14(2):189-202.7. Park D, Jagtap S, Nair S K. Structure of a PL17 Family Alginate Lyase Demonstrates Functional Similarities Among Exotype Depolymerases[J]. Journal of Biological Chemistry, 2014, 289(12).8. Zedong, Jiang, Yuxi, et al. Molecular cloning and characterization of AlgL17, a new exo-oligoalginate lyase from Microbulbifer sp. ALW1.[J]. Protein expression and purification, 2019.9. Wang L, Li S, Yu W, et al. Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.[J]. Biotechnology Letters, 2014, 37(3):665-71.
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