核酸适配体生物传感技术分析牛奶中黄曲霉毒素M1任务书

 2021-10-17 18:54:43

1. 毕业设计(论文)的内容和要求

牛奶中黄曲霉毒素M1残留严重危害人体健康。

目前分析牛奶中黄曲霉毒素M1残留的方法主要集中在仪器分析方法上,成本高、时间长。

本课题拟建立一种核酸适配体生物传感技术分析牛奶中黄曲霉毒素M1残留的方法。

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2. 参考文献

[1] 高晶,袁建振,侯扶江等.饲料及乳制品中黄曲霉毒素的检测[J].草业科学,2018,35(01):222-231.[2] 孙思,王安波,杨梅等.生鲜乳中黄曲霉毒素M1检测技术研究进展[J].畜牧兽医科技信息,2017(04):12-13.[3] 徐琳.鲜牛奶中黄曲霉毒素M1检测的几种方法及控制措施[J].现代畜牧科技,2018(12):99.[4] 罗小虎,王韧,王莉等.高效液相色谱快速测定玉米中黄曲霉毒素的研究[J].中国粮油学报,2014,29(06):99-103.[5] 付春丽,刘文静,高腾云.霉菌毒素对奶牛的影响及其控制措施[J].草业科学,2015,32(09):1500-1507.[6] Henry S H, BoschFX, Bowers J C. Aflatoxin, hepatitis and worldwide liver cancer risks.Advances in Experimental Medicine s milk:Method validation for milk sampled in northern Italy. Toxins, 2016, 8 (3) :57.[12] 马涛.黄曲霉素分析方法简介与比较.科学之友,2007(2):148-148.Ma T.Introduction and comparison of methods for aflatoxin analysis. Friends of Science Amateurs, 2007 (2) :148-148. (in Chinese)[13] Purchase, IFH, etal. TLCdetectedAFM1 atmilkpowders. JAOAC[J], 1967, 50:363-366.[14] 马涛.黄曲霉素分析方法简介与比较.科学之友,2007(2):148-148.Ma T. Introduction and comparison of methods for aflatoxin aflatoxin analysis. Friends of Science Amateurs, 2007 (2) :148-148. (in Chinese)[15] 黄亚伟,魏光,王若兰,罗莉.乳品中黄曲霉毒素M1检测方法研究进展[J].食品安全质量检测学报,2014,5(03):770-775.[16] Omar S S. Aflatoxin M1 levels in raw milk, pasteurised milk and infant formula. Italian Journal of Food Safety, 2016, 5 (3) :158-160.[17] 裴世春,肖理文.基于HRP标记抗体的黄曲霉毒素M1的直接竞争-ELISA[J].食品科学,2011,32(18):221-224.Pei SC, Xiao LW. Rapid Detection of Aflatoxin M1 by Anti-AFM1 mAb-HRP Based Dc-ELISA[J].Food Sci, 2011, 32 (18) :221-224.[18] 廖妍俨,黄家岭.酶联免疫法检测牛奶中黄曲霉毒素M1的应用[J].贵州化工,2012,37(4):33-34.Liao YY, Huang JL. THE application of enzy-linked immunosorbent assay on aflatoxin M1 in milk[J]. Guizhou Chem Ind, 2012, 37 (4) :33-34.[19] 王伟,葛宝坤,周荣等.免疫亲和-荧光光度法快速测定乳脂及其制品中的黄曲霉毒素M1.口岸卫生控制,2006,11(4):24-25.Wang W, Ge B K, Zhou R, Liu X, Su Y, Zhao L J, Wang Z Y. The fast determination of aflatioxin M1in milk grease and its products with immunity affinity and fluorescence photometric method. Port Health Control, 2006, 11 (4) :24-25. (in Chinese)[20] 赵阳阳,吕蕾,刘仁杰等.适配体生物传感器在赭曲霉毒素检测中的应用[J].食品科技,2017,(06):280-284[21] 冯巾,崔承弼,黄宝玺等.适配体生物传感器在黄曲霉毒素检测中的应用[J].食品科技,2018,43(08):336-341.[22] 田育佼.应用消减SELEX技术筛选乙肝表面抗原特异性核酸适配体[D].兰州:兰州大学,2013.Tian YJ. The aptamers of HBsAg were screened by subtractive SELEX[D]. Lanzhou:Lanzhou University, 2013.[23] Yce M, Ullah N, Budak H.Trends in aptamer selection methods and applications[J]. Analyst, 2015, 140(16) :5379-5399.[24] Jing Jing Zhang, Zengmei Li, Shancang Zhao, et al.Size-dependent modulation of graphene oxideaptamer interactions for an amplified fluorescence-based detection of aflatoxin B1 with a tunable dynamic ra nge[J]. Analyst, 2016, 141 (13) :4029-4034[25] Youngung Seok, Ju-Young Byun, Won-Bo Shim, et al. A structure-switchable aptasensor for a flatoxin B1 detection based on assembly of an aptamer/split DNAzyme[J]. Analytica Chimica Acta, 2015, 886:182-187[26] Evtugyn G, Porfireva, Stepanova V, et al.Electrochemical aptasensor based on polycarboxylic macrocycle modified with neutral Red for aflatoxin B1 detection[J]. Electroanalysis, 2014, 26 (10) :2100

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