1. 毕业设计（论文）的内容和要求
1.完成褐藻胶裂解酶的性质表征及作用模式分析；2.利用同源模建及分子对接阐明酶的底物选择性机制；3.完成南京工业大学对于毕业论文的要求。
2. 参考文献
1. Zhu BW, Yin H: Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications. Bioengineered 2015, 6(3):125-131.2. Inoue A, Ojima T: Alginate-modifying enzymes. Nippon Suisan Gakk 2017, 83(5):827-827.3. Ertesvag H: Alginate-modifying enzymes: biological roles and biotechnological uses. Frontiers in Microbiology 2015, 6.4. Kim HS, Lee CG, Lee EY: Alginate Lyase: Structure, Property, and Application. Biotechnol Bioproc E 2011, 16(5):843-851.5. Lombard V, Bernard T, Rancurel C, Brumer H, Coutinho PM, Henrissat B: A hierarchical classification of polysaccharide lyases for glycogenomics. Biochemical Journal 2010, 432:437-444.6. Sim PF, Furusawa G, Teh AH: Functional and Structural Studies of a Multidomain Alginate Lyase from Persicobacter sp CCB-QB2. Sci Rep-Uk 2017, 7.7. Gilbert HJ, Knox JP, Boraston AB: Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules. Curr Opin Struc Biol 2013, 23(5):669-677.8. Zhu BW, Tan HD, Qin YQ, Xu QS, Du YG, Yin H: Characterization of a new endo-type alginate lyase from Vibrio sp W13. International Journal of Biological Macromolecules 2015, 75:330-337.9. Zhu B, Li K, Wang W, Ning L, Tan H, Zhao X, Yin H: Preparation of trisaccharides from alginate by a novel alginate lyase Alg7A from marine bacterium Vibrio sp. W13. International Journal of Biological Macromolecules 2019, 139:879-885.10. Abascal F, Valencia A: Automatic annotation of protein function based on family identification. Proteins 2003, 53(3):683-692.
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