全文总字数：3111字
1. 毕业设计（论文）的内容和要求
恙虫病（tsutsugamushi disease）是恙虫病东方体（即恙虫病立克次体）通过恙螨幼虫叮咬而引起的自然疫源性疾病，临床表现以突发高热、淋巴结肿大、溃疡或皮疹为主。
我国恙虫病疫区呈病例不断增多，扩散的趋势，目前已报道有27个省区均有该病的发生和流行，已成为我国不容忽视的公共卫生疾病。
恙虫病东方体型别不同，毒力也不同，引起人体感染后表现的临床症状差异较大。
2. 实验内容和要求
利用定量PCR方法确定东方体病原接种量，通过动物感染实验和细胞感染实验，计算LD50，比较恙虫病东方体福建分离株和安徽三界分离株的毒力差异，为进一步探索该病原体毒力差异基因奠定基础。
要求熟练掌握掌握分子克隆技术，定量PCR实验，动物及细胞感染实验等。
实验周期3个月，每天在实验室工作不少于8小时
3. 参考文献
1.Paris DH, Phetsouvanh R, Tanganuchitcharnchai A, Jones M, JenjaroenK, Vongsouvath M, Ferguson DP, Blacksell SD, Newton PN, Day NP,Turner GD. 2012. Orientia tsutsugamushi in human scrub typhus eschars shows tropism for dendritic cells and monocytes rather thanendothelium. PLoS Negl Trop Dis 6:e1466.2.Urakami H, Tsuruhara T, Tamura A. 1983. Penetration of Rickettsia tsutsugamushi into cultured mouse fibroblasts (L cells): an electron microscopic observation. Microbiol Immunol 27:251-263. 3.Moron CG, Popov VL, Feng HM, Wear D, Walker DH. 2001. Identification of the target cells of Orientia tsutsugamushi in human cases of scrub typhus. Mod Pathol 14:752-759.4.Urakami H, Tsuruhara T, Tamura A. 1982. Intranuclear Rickettsia tsutsugamushi in cultured mouse fibroblasts (L cells). Microbiol Immunol26:445-447. 5.Rikihisa Y, Ito S. 1982. Entry of Rickettsia tsutsugamushi into polymorphonuclear leukocytes. Infect Immun 38:343-350.6.Fukuhara M, Fukazawa M, Tamura A, Nakamura T, Urakami H. 2005.Survival of two Orientia tsutsugamushi bacterial strains that infect mouse macrophages with varying degrees of virulence. 39:177-187. 7.Cho BA, Cho NH, Seong SY, Choi MS, Kim IS. 2010. Intracellular invasion by Orientia tsutsugamushi is mediated by integrin signaling and actin cytoskeleton rearrangements. Infect Immun 78:1915-1923. 8.Ha NY, Cho NH, Kim YS, Choi MS, Kim IS. 2011. An autotransporterprotein from Orientia tsutsugamushi mediates adherence to nonphagocytic host cells. Infect Immun 79:1718 -1727. 9.Lee JH, Cho NH, Kim SY, Bang SY, Chu H, Choi MS, Kim IS. 2008. Fibronectin facilitates the invasion of Orientia tsutsugamushi into host cells through interaction with a 56-kDa type-specific antigen. J Infect Dis 198:250 -257.10.Ha NY, Sharma P, Kim G, Kim Y, Min CK, Choi MS, Kim IS, Cho NH. 2015.Immunization with an autotransporter protein of Orientia tsutsugamushi provides protective immunity against scrub typhus. PLoS Negl Trop Dis 9:e0003585.11.hn KS, Han SH, Kim HR, Huh MS, Seong SY, Kang JS, Han TH, Kim IS, Choi MS. 2000. Cellular invasion of Orientia tsutsugamushi requires initial interaction with cell surface heparan sulfate. Microb Pathog 28:227-233. 12.Longley RL, Woods A, Fleetwood A, Cowling GJ, Gallagher JT, Couchman JR. 1999. Control of morphology, cytoskeleton and migration by syndecan-4. J Cell Sci 112(Part 20):3421-3431.
4. 毕业设计（论文）计划
2021年1月10日前：查询并阅读相关内容的中外文献和资料2021年1月30日前：在广泛查阅资料的基础上，完善课题设计方案，完成外文翻译。
2021年2月28日前：完成开题报告。
2021年4月15日前：进行课题的实验及结果的处理与分析等。
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