1. 毕业设计（论文）的内容和要求
1. 2. 3. PCR4.
2. 参考文献
1. Cadwallader A B , Yost H J . Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: III. 2-O-sulfotransferase and C5-epimerases[J]. Developmental Dynamics, 2007, 236(2):581-586.2. Wang W , Ju X , Sun Z , et al. Overexpression of heparan sulfate 6-O-sulfotransferase-2 enhances fibroblast growth factor-mediated chondrocyte growth and differentiation[J]. International Journal of Molecular Medicine, 2015.3. Vu T T T , Jeong B , Krupa M , et al. Soluble Prokaryotic Expression and Purification of Human Interferon Alpha-2b Using a Maltose-Binding Protein Tag[J]. Journal of Molecular Microbiology and Biotechnology, 2016:359-368.4. Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides[J]. Angewandte Chemie, 2018, 130(19).5. Thieker D F , Yongmei X , Digantkumar C , et al. Downstream Products are Potent Inhibitors of the Heparan Sulfate 2-O-Sulfotransferase[J]. Scientific Reports, 2018, 8(1):11832-.6. Qianwen L , Tiange C , Yinghong H , et al. A Review of the Structure, Preparation, and Application of NLCs, PNPs, and PLNs[J]. Nanomaterials, 2017, 7(6):122-.7. Meneghetti M C , Gesteira T , Tashima A K , et al. Insights into the role of 3-O-sulfotransferase in Heparan Sulfate biosynthesis[J]. Org. Biomol. Chem. 2017:10.1039.C7OB01533J.8. Rong J , Habuchi H , Kimata K , et al. Substrate Specificity of the Heparan Sulfate Hexuronic Acid 2- O Sulfotransferase 哰J]. Biochemistry, 2001, 40(18):5548-5555.9. Weihua J , Shuai L , Jiale C , et al. Increased soluble heterologous expression of a rat brain 3- O -sulfotransferase 1 ?A key enzyme for heparin biosynthesis[J]. Protein Expression and Purification, 2018, 151:23-29.10. Liu C , Sheng J , Krahn J M , et al. Molecular Mechanism of Substrate Specificity for Heparan Sulfate 2-O-Sulfotransferase[J]. Journal of Biological Chemistry, 2014, 289(19):13407-13418.
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