1. 毕业设计（论文）的内容和要求
课题简介大孔树脂作为一种常用的酶载体材料，能改善酶的活性、选择性、特异性、抗抑制性，甚至可以提高酶的纯度，并且材料本身具有易于修饰改性、表面电荷稳定等特点。
本研究筛选合适大孔树脂作为酶载体，对其进行改性活化以实现糖基转移酶Bs-GT的固定化，并优化固定化条件以期获得更高酶活以及转化率的固定化酶用于生产高附加值产物。
论文要求 1. 文献检索及阅读 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、Elsevier 电子期刊、Springer Link 全文电子期刊等检索资源，查阅关于糖基转移酶研究现状，酶固定化方式优劣，酶固定方式选择及条件优化方面的相关文献。
2. 参考文献
[1] 赵千婧,程瑶,王佳,孙新晓,申晓林,袁其朋.糖基转移酶合成相关糖苷类化合物研究进展[J].北京化工大学学报(自然科学版),2018,45(05):92-99.[2] 金月,吴旭日,陈依军.糖基转移酶在改善天然产物成药性方面的应用[J].中国药科大学学报,2017,48(05):529-535.[3] 金凤燮（主编）.天然产物生物转化[M]. 北京：化学工业出版社，2009:74-101.[4] FEDOROFF N V，FURTEK D B，NELSON O E．Cloning of the bronze locus in maize by a simple and generalizable pocedure using the transposable controlling element activator (ac) [J]． Proc Nati Acad Sci USA，1984，81(12) : 3825－3829.[5] HUGHES J，HUGHES M A.Multiple secondary plant product UDP-glucose glucosyltransferase genes expressed in cassava (Manihot esculenta Crantz) cotyledons[J].[6] SHAO H，HE X，ACHNINE L，et al． Crystal structures of a multifunctional triterpene /flavonoid glycosyltransferase from Medicago truncatula． [J]． Plant Cell，2005，17( 11) : 3141－3154.[7] HAYWARD A P，MORENO M A，HOWARDRT，et al．Control of sexuality by the skl-encoded UDP-glycosytransferase of Maize[J]．Science Advances，2016，2(10) : e16009.[8] XIA T，EITEMAN M A． Quercetin glucoside production by engineered Escherichia coli[J]．Appl Biochem Biotechnol，2017，182(4) : 1358－1370．[9] LUAN L C，PANDEY R P，LIM H N，et al． Synthesis of umbelliferone derivatives in Escherichia coli and their biological activities[J]． Journal of Biological Engineering，2017，11( 1) : 15.[10] PANDEY R P，PARAJULI P，GURUNG R B，et al． Donor specificity of Yjic glycosyltransferase determines the conjugation of cytosolic NDP-sugar in vivo glycosylation reactions[J]． Enzyme ＆ Microbial Technology，2016，91: 26－33．[11] Gutmann A, Nidetzky B. Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5'-Diphosphate-Glucose by Sucrose Synthase[J]. Advanced Synthesis Catalysis, 2016, 358(22):3600-3609.[12] A.C.L.B. Gutmann, A two-step O- to C-glycosidic bond rearrangement using complementary glycosyltransferase activities, CHEMICAL COMMUNICATIONS, 50 (2014) 5465-5469.[13] B.C. Ahn, B.G. Kim, Y.M. Jeon, E.J. Lee, Y. Lim, J.-H. Ahn, Formation of flavone di-O-glucosides using a glycosyltransferase from Bacillus cereus, Journal of microbiology and biotechnology, 19 (2009) 387-390.[14] XU L J，QI T T，XU L，et al． Ｒecent progress in the enzymatic glycosylation of phenolic compounds[J]． Journal of Carbo-hydrate Chemistry，2016，35(1) : 1－23.[15] C. Zhang, B.R. Griffith, Q. Fu, C. Albermann, X. Fu, I.-K. Lee, L. Li, J.S. Thorson, Exploiting the reversibility of natural product glycosyltransferase-catalyzed reactions, Science, 313 (2006) 1291-1294.[16] Wolf S , Berrio R M , Meier C . Synthesis of Nonnatural Nucleoside Diphosphate Sugars[J]. European Journal of Organic Chemistry, 2011, 2011(31):6304-6313.[17] Chem N , April P , Gantt R W , et al. Using Simple Donors to Drive the Equilibria of Glycosyltransferase-Catalyzed Reactions[J]. Nature Chemical Biology, 2011, 7(10):685.[18]梁华正，杨水平，丁志刚，饶军. 弱碱性大孔树脂固定化硫磺菌β- 葡萄糖苷酶的实验研究[J]，食品开发与机械，No. 2. 2008，8-10[19]陈秀琳等，CM-纤维素固定化脂肪酶的研究[J]，海峡药学2005年第17卷第1期
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
