1. 毕业设计（论文）的内容和要求
1. 毕业论文的内容脂肪酶是一类能够催化甘油三酯水解生成脂肪酸、甘油和甘油单酯或二酯的酶，在动植物和微生物体中广泛存在。
通常，脂肪酶对有机溶剂、温度、pH等应用环境较为敏感，导致酶活降低，影响其催化效率。
通过化学修饰来对酶的分子结构进行修饰改造是提升酶学性能的传统手段，课题组前期研究工作证实离子液体作为一种可设计的新型修饰剂可以应用于酶的化学修饰改造，有效提升了酶的催化性能。
2. 参考文献
[1] Jia R, Hu Y, Liu L, et al. Enhancing catalytic performance of Porcine Pancreatic Lipase by covalent modification using functional ionic liquids[J]. ACS Catalysis, 2013, 3(9): 1976-1983.[2] Xu C, Yin X, Zhang C, et al. Improving catalytic performance of Burkholderiacepacia Lipase by chemical modification with functional ionic liquids[J]. Chemical Research in Chinese Universities, 2018, 34(2): 279-284.[3] 熊亚红, 苏健鸿, 刘小平. 邻苯二甲酸酐修饰脂肪酶的性能研究[J]. 华南农业大学学报, 2011, 32(2): 122.[4] Barros M, Fleuri L, Macedo G. Seed lipases: sources, applications and properties - A review[J]. Brazilian Journal of Chemical Engineering, 2010, 27(1): 15 -29.[5] Badgujar K, Pai P, Bhanage B. Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition[J]. Bioprocess and Biosystems Engineering, 2016, 39(2): 211-221.[6] Kharlamov A, Artyushin O and Bondarenko N. Synthesis of some acyclic quaternary ammonium compounds. Alkylation of secondary and tertiary amines in a two-phase system. Russian Chemical Bulletin, 2014, 63(11): 2445-2454[7] 尹燕霞, 向本琼, 佟丽. 荧光光谱法在蛋白质研究中的应用[J].实验技术与管理, 2010, 27(2): 33-36.[8] 郭丽, 徐刚, 吴坚平, 等. 分子模拟指导化学修饰对有机相中脂肪酶的性能强化[J]. 化工进展, 2015, 34(10): 3725-3730.[9] Gilliland G, Berman H, Weissig H, et al.The protein data bank[J]. Nucleic Acids Research, 2000, 28(1): 235-242.[10] Uppenberg J, Patkar S, Bergfors T, et al. Crystallization and preliminary X-ray studies of lipase B from Candida antarctica[J]. Journal of Molecular Biology, 1994, 235(2): 790.[11] Malde K, Zuo L, Breeze M, et al. An automated force field topology builder (ATB) and repository: version 1.0[J]. Journal of Chemical Theory and Computation, 2011, 7(12): 4026-4037.[12] Berendsen H, Spoel D, Drunen R. GROMACS: A message-passing parallel molecular dynamics implementation[J]. Computer Physics Communications, 1995, 91(13): 43-56.[13] Lindahl E, Hess B, Spoel D. GROMACS 3.0: a package for molecular simulation and trajectory analysis[J]. Molecular Modeling Annual, 2001, 7(8): 306-317.[14] Mamashli F, Badraghi J, Delavari B,et al.Improvement of versatile peroxidase activity and stability by a Cheek for cholinium-based ionic liquid[J]. Journal of Molecular Liquids, 2018, 272: 597-608.[15] 赵军, 李雪芹, 修志龙. PEG在线定点修饰水蛭素及其修饰位点的理论预测与分析[J]. 高等学校化学学报,2009, 30(7): 1410-1416. [16] Hu Y, Yang J, Jia R, et al. Chemical modification with functionalized ionic liquids: a novel method to improve the enzymatic properties of Candida rugosa lipase[J]. Bioprocess and Biosystems Engineering, 2014, 37(8): 1617-1626.[17] Mary S, Neda A, Khosro K. Hydrophobic substitution of surface residues affects lipase stability in organic solvents[J]. Molecular Biotechnology, 2014, 56: 360-368.[18] Forde J, Vakurov A, Gibson T, et al. Chemical modification and immobilisation of lipase B from Candida antarctica onto mesoporous silicates[J]. Journal of Molecular Catalysis B: Enzymatic, 2010, 66(1): 203-209.[19] Itoh T. Ionic liquids as tool to improve enzymatic organic synthesis[J]. Chemical Reviews, 2017, 117(15): 10567-10607.[20] Wahl J, Holzgrabe U. Capillary electrophoresis separation of phenethylamine enantiomers using amino acid based ionic liquids[J]. Journal of Pharmaceutical and Biomedical Analysis, 2018, 148: 245-250.
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