1. 毕业设计（论文）的内容和要求
内容和要求1.文献查阅学会利用计算机在中国期刊网、维普数据网、Springerlink、CA、SCI、Elsevier等等网络检索资源上检索、查阅与二氢乳清酸酶及其应用相关的文献，掌握文献查阅的方法。
2.文献阅读及综述仔细阅读与所做课题相关的国内外文献，了解相关的背景知识以及目前国内外的研究动态，会系统分析，撰写文献综述。
3.明确实验任务，拟定实验方案根据所查文献的内容，结合大学所学课程相关知识，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
[1] Syldatk C，May O，Altenbuchner J，Mattes R，Siemann M，Microbial hydantoinases-industrial enzymes from the origin of life[J]. Appl. Micorbiol. Biotechnol. 1999,51: 293-309.[2] Holm L and Snader C. An evolutionary treasure: unification of a broad set of amidohydrolases related to urease [J]. Proteins .1997, 28: 72-82.[3] Meng Z，Yamazaki T. Construction of artificial cyclic amide amidohydrolases using molecular imprinting technique[J].Biotechnol Lett，2005, 27(7): 471-475.[4] Baldwin，J.，Michnoff，C.H.，Malmquist N. A，White，J.，Roth，M. G, Rathod，P.K. and Philips, M. A.High-throughput screening for potent and selective inhibitoes of Plasmodium falciparum dihydroorotate dehydrogenase [J]. Journal of Biological Chemistry. 2005,36: 585-589. [5]Miller S, Lansdale, Edward. Dihydroorotic acid [J]. United States Patent Office. 1954, 12: 260-260[6] Tamiko N. Porter, Yingchun Li, and Frank M. Raushel, Mechanism of the Dihydroorotase Reaction [J], Biochemistry. 2004, 43:16285-16292.[7] Patricia E. Schroeder, Jeffrey N. Davidson, and Brian B. Hasinoff, Dihydroorotase catalyzes the ring opening of the hydrolysis ntermediates of the cardioprotective drug dexrazoxane (ICRF-187), [J]. Faculty of Pharmacy.2002,30: 14311435.[8]Huang, D. T., Kaplan, J., Menz, R. I., Katis, V. L., Wake, R. G.,Zhao, F., Wolfenden, R., and Christopherson, R. I. Thermodynamic analysis of catalysis by the dihydroorotases from hamster and Bacillus caldolyticus, as compared with the uncatalyzed reaction, [J].Biochemistry.2006, 45:8275-8283.[9] Huang, D. T., Thomas, M. A., and Christopherson, R. I. Divalent metal derivatives of the hamster dihydroorotase domain, [J].Biochemistry. 1999, 38: 9964-9970.[10]蒋兴仁，阿斯巴甜简介,[J].食品工业科技，1989（3）：42-45.[11]冷一欣等，N-甲酰-L-天冬氨酸酐的合成，[J].精细化工，2001, 18：50-52.[12]Hijuchic, kato, Ora. T Preparation of alapha-L-asparty-L-phenylalanie ester as low-calorie swrrtner[P] JP:1991-255093,1991-11-13, (CA1992,116;1296266)[13]樊可可，韦萍等，两相溶剂体系酶促合成Asparatame前体的研究[J]高校化学工程学报，1999,4:382-385[14]许激杨等，酶法合成Asparatame的研究[J]药物生物技术，1994,1,44-45[15]张红缨等，α-天冬氨酰二肽酶基因的高表达和产物回收，[J]生物工程学报，1998,2:208-213.[16]王晓平等，α-天冬氨酰二肽酶基因工程菌的固定化及其应用，[J]吉林大学自然科学学报，1999,2:85-87.[17] 宋芸, 魏绍川, 王永峰. 瑞林类寡肽抗癌药物的合成研究进展 [J] 合成化学, 2010 18(2): 141-147.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
