1. 毕业设计（论文）的内容和要求
熟悉实验室重要仪器的使用原理和常见问题的处理方法；系统了解文献查阅过程，并熟悉掌握各种数据库的使用方法；了解蛋白质组学研究的相关技术；熟练掌握产环磷酸腺苷的发酵过程；熟练掌握微生物发酵实验的相关操作，能够熟练的配制斜面培养基、种子液、发酵液，熟悉无菌概念以及操作要点；熟练掌握pH计的使用方法，紫外可见分光光度计的操作和高效液相色谱检测方法；考察不同溶氧条件对产物产量的影响；精心设计实验，认真规范操作，并即使处理数据，实事求是。
2. 参考文献
[1] Cheng, J. S., X. Zhou, M. Z. Ding, and Y. J. Yuan (2009) Proteomic insights into adaptive responses of Saccharomyces cerevisiae to the repeated vacuum fermentation. Applied microbiology and biotechnology. 83: 909-923.[2] Li, B.-Z., and Y.J. Yuan (2010) Transcriptome shifts in response to furfural and acetic acid in Saccharomyces cerevisiae. Applied microbiology and biotechnology. 86: 1915-1924.[3] Lin, F. M., B. Qiao, and Y.J. Yuan (2009) Comparative proteomic analysis of tolerance and adaptation of ethanologenic Saccharomyces cerevisiae to furfural, a lignocellulosic inhibitory compound. Applied and environmental microbiology. 75: 3765-3776.[4] Ma, Q., J. Zhou, W. Zhang, X. Meng, J. Sun, and Y.J. Yuan (2011) Integrated proteomic and metabolomic analysis of an artificial microbial community for two-step production of vitamin C. PloS one. 6: e26108.[5] Huang G. D., C. B. Li, and Y. S. Cao. (2011) Proteomic analysis of direntially expressed proteins in Lactobacillus brevis NCL912underacid stress. FEMS Microbiol Lett. 318: 177182.
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