1. 毕业设计（论文）的内容和要求
一、 论文内容： 随着分子生物学的发展，科学家们对生物体的了解越发深入。
基因工程的开发使得单细胞生物能够异源表达基因，从而实现功能的转移。
而新兴的合成生物学，通过代谢工程对底盘细胞的代谢途径进行设计、构建以及优化，从而实现了绿色环保的生物制造，例如保健品、药物、生物燃料以及各种精细化学品和大宗化学品。
2. 参考文献
[1] 武耀康, 刘延峰, 李江华. 动态调控元件及其在微生物代谢工程中的应用[J]. 化工学报, 2018, v.69(01):280-289.[2] Nielsen J, Keasling J. Engineering Cellular Metabolism[J]. Cell, 2016, 164(6):1185-1197.[3] Liu J, Li J, Shin H.D., et al. Protein and metabolic engineering for the production of organic acids[J]. Bioresource Technology, 2017, S0960852417305424.[4] Keasling J.D. Synthetic biology and the development of tools for metabolic engineering[J]. Metabolic Engineering, 2012, 14(3):189-195.[5] Nicolson G.L. Temperature-dependent control over the dynamic display of surface receptors and the fluid mosaic membrane model[J]. Cryobiology, 1975, 12(6):1.[6] Allison H.O, Kristina H, Lukas K, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, (12):12.[7] Lipfert J, Das R, Chu V.B, et al. Structural Transitions and Thermodynamics of a Glycine-Dependent Riboswitch from Vibrio cholerae[J]. Journal of Molecular Biology, 2007, 365(5):0-1406.[8] Chen, Xiulai, Liu, et al. Gene Circuits for Dynamically Regulating Metabolism[J]. Trends in biotechnology, 2018.[9] 徐九丁, 童葵塘. 温敏工程菌的表达与调控[J]. 国际生物制品学杂志, 1993(2).[10] Xiaolin, Shen, Jia, et al. Dynamic gene expression engineering as a tool in pathway engineering[J]. Current opinion in biotechnology, 2019.[11] Xu, Peng. Production of chemicals using dynamic control of metabolic fluxes[J]. Current Opinion in Biotechnology, 2018, 53:12-19.[12] Zhou P, Li M, Shen B, et al. Directed co-evolution of β-carotene ketolase and hydroxylase and its application in temperature-regulated biosynthesis of astaxanthin[J]. Journal of Agricultural and Food Chemistry, 2019.[13] Venayak N, Anesiadis N, Cluett W.R, et al. Engineering metabolism through dynamic control[J]. Current Opinion in Biotechnology, 2015, 34:142-152.
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