1. 毕业设计（论文）的内容和要求
课题简介酶活性口袋特性是决定酶催化效率和特异性的主要因素。
酶EG5C-1属于GH5家族，本研究以高相似性的枯草芽孢杆菌168的内切酶BsCel5A，为模板建立了酶EG5C-1的三维结构模型，该酶呈现典型的（β/α）8桶状结构，活性口袋大小为31A，能容纳的糖分子为纤维六糖。
通常认为酶活性口袋中与底物直接作用的氨基酸残基与底物分子的距离应该小于5A，通过酶-底物对接模拟分析表明，酶EG5C-1中共有23个氨基酸残基与纤维六糖存在直接作用，我们选取其中的芳香族氨基酸对其进行深入研究，以提高其相对的活性。
2. 参考文献
1.Wu B , Zheng S , Pedroso M M , et al. Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates[J]. Biotechnology for Biofuels, 2018, 11(1):20.2.Cao L , Li S , Huang X , et al. Enhancing the thermostability of highly active and glucose-tolerant β-glucosidase Ks5A7 by directed evolution for good performance on three properties[J]. Journal of Agricultural and Food Chemistry, 2018.3.Ibrahim, Torktaz, Ali, et al. Rational engineering of Cel5E from Clostridium thermocellum to improve its thermal stability and catalytic activity.[J]. Applied microbiology and biotechnology, 2018.4.Lin L , Fu C , Huang W . Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR[J]. Enzyme and Microbial Technology, 2016, 86:52-58.5.Ma M , Okuyama M , Sato M , et al. Effects of mutation of Asn694 in Aspergillus niger α-glucosidase on hydrolysis and transglucosylation[J]. Applied Microbiology and Biotechnology, 2017, 101(16):1-10.6.Zheng F , Tu T , Wang X , et al. Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6[J]. Biotechnology for Biofuels, 2018, 11(1):76.7.Jing-Jing, Chen, Xiao, et al. Site-Directed Mutagenesis of a β-Glycoside Hydrolase from Lentinula Edodes.[J]. Molecules (Basel, Switzerland), 2018.8.Li, Y, Irwin, D. C, Wilson, D. B. Increased Crystalline Cellulose Activity via Combinations of Amino Acid Changes in the Family 9 Catalytic Domain and Family 3c Cellulose Binding Module of Thermobifida fusca Cel9A[J]. Applied Environmental Microbiology, 76(8):2582-2588.9.Guo B , Amano Y , Nozaki K . Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis[J]. PLOS ONE, 2016, 11(1):e0147301.Ibrahim, Torktaz, Ali, et al. Rational engineering of Cel5E from Clostridium thermocellum to improve its thermal stability and catalytic activity.[J]. Applied microbiology and biotechnology, 2018.
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