1. 毕业设计（论文）的内容和要求
（1）论文内容本实验采用基因工程手段，挖掘筛选优势植物来源的α-糖苷酶基因序列，采用胞外分泌表达策略构建重组表达质粒。
以毕赤酵母GS115为宿主诱导表达产酶，以麦芽糖为底物，研究α-糖苷酶的催化性能。
（2）论文要求1．文献查阅掌握文献查阅的一般方法，学会利用互联网在中国期刊网、维普数据库、超星数字图书馆、Web of Science科学引文索引、Springer Link电子期刊、Elsevier SDOS电子期刊等检索资源上查阅关于糖基转移酶基因及催化合成各类天然产物的相关文献，对本研究内容有广泛了解。
2. 参考文献
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Applied and Environmental Microbiology, 2013, 79(2): 672-677.[21] Liu L, Han R Z, Shin H D, et al. Improving maltodextrin specificity for enzymatic synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid by site-saturation engineering of subsite-3 in cyclodextrin glycosyltransferase from Paenibacillus macerans [J]. Journal of Biotechnology, 2013, 166(4): 198-205.[22] Chen S, Xiong Y J, Su L G, et al. Position 228 in Paenibacillus macerans cyclodextrin glycosyltransferase is critical for2-O-D-glucopyranosyl-L-ascorbic acid synthesis [J]. Journal of Biotechnology, 2017, 247: 18-24. [23] Tao X M, Wang T, Su LQ, et al. Enhanced 2-O-alpha-D-glucopyranosyl-L-ascorbic acid synthesis through iterative saturation mutagenesis of acceptor subsite residues in Bacillus stearothermophilus NO2 cyclodextrin glycosyltransferase [J]. Journal of Agricultural and Food Chemistry, 2018,66(34): 9052-9060.[24] Tai A, Iomori A, Ito H. 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