1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、Elsevier电子期刊、Springer Link全文电子期刊等检索资源，查阅关于环糊精葡萄糖苷转移酶的相关文献。
2．文献阅读及综述阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
1: Menendez-Bravo S, Comba S, Gramajo H, Arabolaza A. Metabolic engineering ofmicroorganisms for the production of structurally diverse esters. AppliedMicrobiology and Biotechnology. 2017, 101:3043-3053.2: 王海京, 段启伟. 正丁醇一步合成丁酸丁酯. 精细石油化工. 2003, 第 1 期.3: Rhodri WJ, Martin M, Sarah N, Christopher JC. Potential renewable oxygenatedbiofuels for the aviation and road transport sectors. Fuel. 2013, 103:593-599.4: Yi-Shu T, Mingyong X, Kechun Z. Engineering biosynthesis of medium-chainesters in Escherichia coli. Metabolic Engineering. 2015, 11:20-28.5: Adachi S, Kobayashi T. Synthesis of esters by immobilized-lipase catalyzedcondensation reaction of sugars and fatty acids in water miscible organic solvent.Journal of Bioscience and Bioengineering. 2005, 99:87-94.6: Schiel-Bengelsdorf B, Montoya J, Linder S, Drre P. Butanol fermentation.Environmental Technology. 2013, 34:1691-710.7: Xin F, Yan W, Zhou J, Wu H, Dong W, Ma J, Zhang W, Jiang M. Exploitation ofnovel wild type solventogenic strains for butanol production. Biotechnology forBiofuels. 2018, 11:252.8: Xin F, Dong W, Jiang Y, Ma J, Zhang W, Wu H, Zhang M, Jiang M. Recentadvances on conversion and co-production of acetone-butanol-ethanol into highvalue-added bioproducts. Critical Reviews in Biotechnology. 2018, 38:529-540.9: Horton CE, Bennett GN. Ester production in E. coli and C. acetobutylicum.Enzyme and Microbial Technology. 2006, 38:937-943.10: Noh HJ, Woo JE, Lee SY, Jang YS. Metabolic engineering of Clostridiumacetobutylicum for the production of butyl butyrate. Applied Microbiology andBiotechnology. 2018, 102:8319-8327.11: van den Berg C, Heeres AS, van der Wielen LA, Straathof AJ. Simultaneousclostridial fermentation, lipase-catalyzed esterification, and ester extraction toEnrich diesel with butyl butyrate. Biotechnology and Bioengineering. 2013,110:137-142.12: Xin F, Basu A, Yang KL, He J. Strategies for production of butanol andbutyl-butyrate through lipase-catalyzed esterification. Bioresource Technology.2016, 202:214-219.13: Seo SO, Wang Y, Lu T, Jin YS, Blaschek HP. Characterization of a Clostridiumbeijerinckii spo0A mutant and its application for butyl butyrate production.Biotechnology and Bioengineering. 2017, 114:106-112.14: Zhang ZT, Taylor S, Wang Y. In situ esterification and extractive fermentation forbutyl butyrate production with Clostridium tyrobutyricum. Biotechnology andBioengineering. 2017, 114:1428-1437.15: Xin F, Liu J, He M, Wu B, Ni Y, Dong W, Zhang W, Hu G, Min J. Highbiobutanol production integrated with in situ extraction in the presence of Tween80 by Clostridium acetobutylicum. Process Biochemistry. 2018, 67:113-117.16: Gu Y, Jiang Y, Yang S, Jiang W. Utilization of economical substrate-derivedcarbohydrates by solventogenic clostridia: pathway dissection, regulation andengineering. Current Opinion in Biotechnology. 2014, 29:124-131.17: Patakova P, Kolek J, Sedlar K, Koscova P, Branska B, Kupkova K, Paulova L,Provaznik I. Comparative analysis of high butanol tolerance and production inclostridia. Biotechnology Advances. 2018, 36:721-738.18: Xue C, Zhao J, Chen L, Yang ST, Bai F. Recent advances and state-of-the-artstrategies in strain and process engineering for biobutanol production byClostridium acetobutylicum. Biotechnology Advances. 2017, 35:310-322.19: Lv J, Jiao S, Du R, Zhang R, Zhang Y, Han B. Proteomic analysis to elucidatedegeneration of Clostridium beijerinckii NCIMB 8052 and role of Ca(2 ) in strainrecovery from degeneration. Journal of Industrial Microbiology Biotechnology.2016, 43:741-750.20: Cornillot E, Nair RV, Papoutsakis ET, Soucaille P. The genes for butanol andacetone formation in Clostridium acetobutylicum ATCC 824 reside on a largeplasmid whose loss leads to degeneration of the strain. Journal of Bacteriology.1997, 179:5442-5447.21: Wen Z, Minton NP, Zhang Y, Li Q, Liu J, Jiang Y, Yang S. Enhanced solventproduction by metabolic engineering of a twin-clostridial consortium. MetabolicEngineering. 2017, 39:38-48.22: Wang C, Xin F, Kong X, Zhao J, Dong W, Zhang W, Ma J, Wu H, Jiang M.Enhanced isopropanol-butanol-ethanol mixture production through manipulation ofintracellular NAD(P)H level in the recombinant Clostridium acetobutylicum XY16.Biotechnology for Biofuels. 2018, 11:12.23: Zhang J, Yu L, Xu M, Yang ST, Yan Q, Lin M, Tang IC. Metabolic engineering ofClostridium tyrobutyricum for n-butanol production from sugarcane juice. AppliedMicrobiology and Biotechnology. 2017, 101:4327-4337.24: Xin F, He J. Characterization of a thermostable xylanase from a newly isolatedKluyvera species and its application for biobutanol production. BioresourceTechnology. 2013, 135:309-315.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
