1. 毕业设计（论文）的内容和要求
产琥珀酸放线杆菌是天然的丁二酸生产菌株，作为细菌由于其较差的pH耐受性，造成后续丁二酸分离难度增加，且为维持稳定的pH条件，中和剂的添加，也会造成渗透压的增加，加重菌株的负荷。
有文献表明，细菌在较低pH条件下其细胞膜的结构会发生变化，并且有利于细胞的生长和丁二酸的生产，本论文就获得高pH耐受性的产琥珀酸放线杆菌，从NJ113出发，在低pH的选择压力下，驯化得到对pH有良好耐受条件的产琥珀酸放线杆菌，从而提高丁二酸的产量。
2. 参考文献
1 M. Anitha, S.K. Kamarudin, N.T. Kofli. The potential of glycerol as a value-added commodity. ChemicalEngineering Journal. 2016;295:119-30.2 M.J. Van der Werf, M.V. Guettler, M.K. Jain, J.G. Zeikus. Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Arch Microbiol. 1997;167(6):332-42.3 B.D. Schindler, R.V. Joshi, C. Vieille. Respiratory glycerol metabolism of Actinobacillus succinogenes 130Z for succinate production. J Ind Microbiol Biot. 2014;41(9):1339-52.4 M. Carvalho, M. Matos, C. Roca, M.A. Reis. Succinic acid production from glycerol by Actinobacillus succinogenes using dimethylsulfoxide as electron acceptor. N Biotechnol. 2014;31(1):133-9.5 G. Durnin, J. Clomburg, Z. Yeates, P.J. Alvarez, K. Zygourakis, P. Campbell, R. Gonzalez. Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli. Biotechnol Bioeng. 2009;103(1):148-61.6 H.J. Hwang, S.P. Choi, S.Y. Lee, J.-i. Choi, S.J. Han, P.C. Lee. Dynamics of membrane fatty acid composition of succinic acid-producing Anaerobiospirillum succiniciproducens. Journal of Biotechnology. 2015;193:130-3.7 A.W. Kingston, C. Subramanian, C.O. Rock, J.D. Helmann. A sigmaW-dependent stress response in Bacillus subtilis that reduces membrane fluidity. Mol Microbiol. 2011;81(1):69-79.8 P. Luly, P. Baldini, S. Incerpi, E. Tria. Insulin effect in vitro on human erythrocyte plasma membrane. Experientia. 1981;37(4):431-3.9 T.D. Harrington, V.N. Tran, A. Mohamed, R. Renslow, S. Biria, L. Orfe, D.R. Call, H. Beyenal. The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction. Bioresource technology. 2015;192:689-95.
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