全文总字数：1876字
1. 毕业设计（论文）的内容和要求
1. 严格按照实验室安全规则进行，佩戴护目镜、手套、实验服等；2. 熟悉实验涉及的每种化合物的物理化学性质，确保实验顺利进行；3. 熟练掌握细菌培养操作技术；4. 熟练掌握还原反应操作过程。
2. 实验内容和要求
1、认识烯还原酶催化还原反应的机理；2、熟悉烯还原酶培养、纯化等基础流程；3、掌握酶催化反应流程；4、合成5-15种该课题所需还原产物。
3. 参考文献
1. Williams RE, Bruce NC: 慛ew uses for an Old Enzyme??the Old Yellow Enzyme family of flavoenzymes. Microbiology 2002, 148:1607.2. Scholtissek A, Tischler D, Westphal AH, van Berkel WJH, Paul CE: Old Yellow Enzyme-catalysed asymmetric hydrogenation:linking family roots with improved catalysis. Catalysts 2017:7.3. Stuermer R, Hauer B, Hall M, Faber K: Asymmetric bioreduction of activated C糃 bonds using enoate reductases fromthe Old Yellow Enzyme family. Curr Opin Chem Biol 2007,11:203.4. Faber K, Hall M: Addition of hydrogen to C糃 double bonds: alkene reduction. In Science of Synthesis: Biocatalysis in Organic Synthesis. Edited by Faber K, Fessner WD, Turner NJ.Stuttgart, Germany: Georg Thieme Verlag; 2015:213-260.5. Durchschein K, Hall M, Faber K: Unusual reactions mediated by FMN-dependent ene- and nitro-reductases. Green Chem 2013,15:1764.6. Karplus PA, Fox KM, Massey V: Structure杅unction relations for Old Yellow Enzyme. FASEB J 1995, 9:1518.7. Strassner J, Schaller F, Frick UB, Howe GA, Weiler EW, Amrhein N, Macheroux P, Schaller A: Characterization and cDNAmicroarray expression analysis of 12-oxophytodienoatereductases reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response. Plant J 2002, 32:585.8. Jakubczyk D, Caputi L, Hatsch A, Nielsen CAF, Diefenbacher M, Klein J, Molt A, Schroder H, Cheng JZ, Naesby M et al.: Discoveryand reconstitution of the cycloclavine biosynthetic pathwayenzymatic formation of a cyclopropyl group. Angew Chem Int Ed 2015, 54:5117.
4. 毕业设计（论文）计划
2021年1月-2021年2月文献阅读2021年2月-2021年5月集中实验操作2021年5月-2021年6月集中论文撰写
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
